超临界CO_2萃取烟叶中茄尼醇的工艺研究

Extraction of Solanesol from Tobacco Leaves by Supercritical Fluid CO_2

采用超临界二氧化碳萃取烟叶中的茄尼醇,考察了萃取过程温度、压力、二氧化碳流量、萃取方式、夹带剂种类及其用量对萃取过程的影响。采用四因素三水平的正交设计,以茄尼醇的提取率为指标,对萃取条件进行了优化。并建立了能较准确测定烟叶中游离茄尼醇含量的方法,采用高效液相色谱对萃取产物进行了分析。以探索提高提取率的方法,为其工业化生产提供参考。结果表明:最佳萃取工艺条件为:甲醇为夹带剂,萃取温度45℃,萃取压力25MPa,CO2流量15kg·h-1。在最佳工艺条件下茄尼醇的提取率为:92.1%。

The process for solanesol extracting from tobacco leaves with Supercritical Fluid CO2 was studied. The factors affecting the process were researched including temperature, pressure, the flow-rate of CO2, the manner of extraction, entrainers and its adding manner. The optimum extraction conditions of solanesol were investigated by orthogonal design of four factors and three levels with the goal of solanesal extraction yield. It was found for a more accurate determination method of free solanesol in tobacco leaves and the extracted anthraquinones were analysed by high-performance liquid chromatography （HPLC）. It was the goal to study the method of improving the extraction yield of solanesol, and provide certain guidance of the industrialized production. The results show that the optimum extracting parameters were： methanol as entrainer, the flow-rate of CO2 at 15 kg· h^-1, temperature at 45℃ and pressure under 25 MPa, Under optimal extraction conditions, the extraction yield of solanesol was 92. 1%.