熟地黄粗多糖对血虚模型小鼠胸腺和脾脏组织形态的影响

Effects of Gross Polysaccharide of Radix Rehmanniae Preparata on Morphology Changes of Thymus and Spleen in Blood Deficiency Model Mice

目的:探讨熟地黄粗多糖对血虚模型免疫器官形态的影响。方法:以放血与环磷酰胺并用致小鼠血虚模型为研究对象,将模型动物分为5组,在造模的同时给药,分别灌服大、中、小剂量的熟地黄粗多糖溶液、当归补血口服液和同体积生理盐水,另设一组空白对照组,每天给药1次,连续给药10 d,实验结束时取胸腺和脾脏作病理切片。结果:熟地黄粗多糖组可显著对抗造模所致动物胸腺和脾脏的萎缩,显著增加模型动物胸腺皮质厚度和皮质细胞数,显著增加脾小结大小和皮质细胞数。结论:怀熟地黄多糖可拮抗血虚模型小鼠胸腺及脾脏的萎缩。

Objective： To probe the effects of gross polysaccharide of Radix Rehmanniae Preparata （GPRRP） on the morphology changes of immune organ of blood deficiency model mice. Methods： Using the blood deficiency mice models induced by releasing blood and injection of Cyclophosphamide as the experimental objects, the model animals were divided into 5 groups, that was the large, middle and small dose of GPRRP groups, Danggui Buxue Oral Liquid group, model group, at the same time, giving a blank control group. It was given successively relating drugs to the animals of several groups for 10 days. At the end of the experiment, the hemogram was measured and the thymus and spleen were taken, and making pathological section, then these tissues morphology changes were ob- served under microscope. Results： GPRRP can obviously resist the atrophy of thymus and spleen induced by the model establishing, can obviously increase the depth of thymus cortex and the number of cortex cells, can enlarge the size of spleen nodules and obviously increase the number of lymphocyte. Conclusion： GPRRP can resist the atrophy of thymus and spleen in blood deficiency model mice.