足月胎盘单核-巨噬细胞体外诱导分化为树突状细胞

Monocyte-macrophages separated from the normal term differentiate into functional dendritic cells in vitro

目的:研究人类足月胎盘中的单核-巨噬细胞是否能分化为树突状细胞(DCs)。方法:机械分离足月胎盘中的单核-巨噬细胞,加入内皮细胞单层中,经正逆两次穿越内皮,48h后收集返回到内皮单层上方得到的细胞,以扫描电子显微镜和普通光学显微镜观察细胞形态,流式细胞仪分析细胞表型及3H-TdR检测DCs激发异体T细胞增殖能力。结果:足月胎盘中的单核-巨噬细胞经过(逆)穿越内皮系统后,具有典型的DCs的形态学特征,相对于胎盘单核-巨噬细胞而言高表达CD80、CD86以及HLA-DR,并具有激发异体T细胞增殖能力。结论:足月胎盘中的单核巨噬细胞在内皮(逆)穿越系统中可以分化为DCs。

Objective：To investigate whether monocyte-macrophages separated from the normal term placenta could differentiate into functional dendritic cells （DCs） in vitro. Methods：Mechanically separated monocyte-macrophages from normal term placenta were cultured in the transendothelial trafficking model for 48 h. The DCs induced from placental monocyte-macrophages were identified for their morphology and phenotype by scanning electron microscope, FACS and for their abilities of inducing proliferation of allogenic T cells by 3H-TdR incorporation assay. Results： After cultured in the transendothelial trafficking model for 48 h, the DC-like cells derived from the normal term placental monocyte-macrophages displayed characteristic morphology and expressed higher level surface antigen of CD80, CD86 and HLA-DR, and could stimulate the proliferation of allogenic T cells. Conclusion：In the transendothelial trafficking model monocyte-macrophages separated from the normal term placenta can be induced into functional dendritic cells（DCs）.