支气管哮喘患儿外周血白细胞介素19水平及其临床意义

The clinical value of the Interleukin-19 level in peripheral blood of children patients with bronchial asthma

目的:检测哮喘患儿外周血白介素19(IL-19)水平,探讨其临床意义。方法:实验分3组,发作期组(21例)、缓解期组(19例)、正常对照组(22例)。采用酶联免疫吸附试验(ELISA)法检测外周血IL-19、IgE水平,血细胞分析仪计数外周血嗜酸性粒细胞(EOS)。结果:与对照组比较,发作期组和缓解期组外周血IL-19、IgE水平和EOS明显增高(P<0.01)。发作期组IL-19和EOS水平高于缓解期组(P<0.05),而发作期组IgE水平与缓解期组比较差异无显著性。相关性分析显示,外周血IL-19与IgE及EOS均呈正相关(r=0.837,P<0.01;r=0.742,P<0.01)。结论:外周血IL-19在哮喘不同时期上升程度不同。IL-19可能在哮喘发病机制中发挥重要作用,观察其变化有助于哮喘临床诊断和病情监测。

Objective：To study the level of interleukin 19 （IL-19）in peripheral blood of children with asthma and investigate its importance. Methods：Three groups were in the experiment：the acute onset group of asthma （21 patients）, the catabasis group of asthma（19 patients）, and health control group（22 cases ）.The level of IL-19 and IgE in peripheral blood was respectively determined with ELISA. The accurate number of EOS in peripheral blood was counted with cytoanalyze. Results：Compared with the children of control group, the levels of IL-19, IgE and the quantity of EOS in peripheral blood of the patients with Asthma were obvious higher（P 〈 0.01）.The level of IL-19 and the quantity EOS of the patients of acute attack group were higher than those of the patients of catabasis group（P 〈 0.05）, but there is no significant difference in the level of IgE between the two groups of asthma. Furthermore, the results of correlation analysis showed that the levels of IL-19 positively correlated to the level of IgE and the quantity of EOS respectively in blood （r = 0.837, P 〈 0.01 ;r = 0.742, P 〈 0.01 ）. Conclusion：The IL-19 level of the children with asthma was always higher than that of health children, and the ascending degree of the IL-19 level are different during various periods of asthma. It may be inferred that IL-19 can play an important role in the pathogenesis of asthma, and observing the change of the level of IL-19 can make for the clinical diagnosis and monitoring the pathogenetic condition of asthma.