染料木黄酮对人乳腺癌细胞肌动蛋白细胞骨架和黏附能力的影响

Effects of genistein on the actin cytoskeleton and the adhesion of human breast cancer cells

目的:体外研究染料木黄酮(genistein)对人乳腺癌细胞(MDA-MB-435)肌动蛋白细胞骨架及黏附能力的影响。方法:不同浓度染料木黄酮(12.5、25.0、50.0和100.0!mol/L)孵育MDA-MB-435细胞24h。用FITC标记的鬼笔环肽标记F-肌动蛋白,分别用荧光显微镜和流式细胞仪技术分析肌动蛋白细胞骨架的重组情况;噻唑蓝比色法(MTT)检测细胞对人工重组基膜的黏附能力。结果:不同浓度染料木黄酮均导致细胞内肌动蛋白细胞骨架发生解聚,同时细胞内F-肌动蛋白排列和分布及细胞形态发生明显改变;此外,染料木黄酮可明显降低MDA-MB-435细胞对人工重组基膜的黏附能力,其效应呈剂量依赖性。结论:染料木黄酮在体外可抑制乳腺癌细胞的黏附能力,其作用可能与其诱导肌动蛋白细胞骨架重组有关。

Objective：To investigate the effects of genistein on the the actin cytoskeleton and the adhesion of human breast cancer cells（MDA-MB-435 cells）. Methods：The MDA-MB-435 cells were treated with different concentrations of genistein（ 12.5, 25.0, 50.0 and 100.0 μmol/L） for 24 h. Cellular F-actin stained with FITC-phalloidin was examined by fluorescence microscopy and flow cytometric analysis. The adhesive ability of MDA-MB-435 cells to artificial basement membrane （Matrigel） was measured by MTT assay. Results：Genistein significantly induced the depolymerization of cellular F-actin and caused the changes of cellular morphology and the redistribution of the F-actin. Meanwhile, genistein also reduced the adhesion of MDA-MB-435 cells to Matrigel. Conclusion： Genistein induced the less adhesion of MDA-MB-435 cells to Matrigel, which may related to the reorganization of actin cytoskeleton.