摘要
对沈抚灌区水改旱田不同年限土壤的石油污染物浓度及组分进行了分析,并采用变性梯度凝胶电泳和磷脂脂肪酸分析方法,分析了污染土壤微生物群落结构的变化.结果表明:1)石油污染土壤水改旱田后,年限越长,总多环芳烃在总石油烃中所占的比重越大,高分子量多环芳烃在总多环芳烃中所占比重也越大;2)总磷脂脂肪酸量与总石油烃呈显著正相关,与总多环芳烃相关性不显著;3)两种方法对土壤微生物群落结构的分析得出的结论一致,石油污染土壤微生物群落结构主要与其相对地理位置有关,当污染物的浓度达到一定程度时,土壤微生物群落结构会发生明显的改变.
With the oil-polluted soils after converted from paddy field to upland field for different years in the Shenfu wastewater irrigation area of Shenyang as test materials, the concentrations of their oil pollutants were determined, and the microbial community structure was investigated by de- naturing gradient gel electrophoresis （DGGE） and phospholipids fatty acid （PLFA） methods. The results showed that the proportions of total polynuclear aromatic hydrocarbons （PAHs） in total petroleum hydrocarbons （TPHs） and of high molecular weight PAHs in total PAHs increased with the increasing years of field conversion. Total phospholipid fatty acids （PLFAs） had a significant positive correlation with total TPHs, but no correlation with total PAHs. Both DGGE and PLFA analyses indicated that the community structure of soil microbes was mainly correlated with geographical location, and changed obviously when the concentrations of oil pollutants in soil increased to a definite level.
出处
《应用生态学报》
CAS
CSCD
北大核心
2007年第5期1107-1112,共6页
Chinese Journal of Applied Ecology
基金
国家自然科学基金项目(30670391)
国家重点基础研究发展规划资助项目(2004CB418505).
关键词
污水灌溉
微生物多样性
磷脂脂肪酸
变性梯度凝胶电泳
总石油烃
多环芳烃
wastewater irrigation
microbial diversity
phospholipid fatty acid （PLFA）
denaturing gradient gel electrophoresis （ DGGE ）
total petroleum hydrocarbon （TPH）
polynuclear aro- matic hydrocarbon （PAH）.
