温脉通对高脂致血管内皮细胞损伤的保护作用

Protective effect of Wenmaitong on vascular endothelial cells from injury induced by hyperlipidemic serum in vitro

目的研究温脉通对高脂损伤血管内皮细胞的保护作用,以探讨其治疗动脉硬化闭塞症(ASO)的机制。方法用高脂血清造成人脐静脉内皮细胞(HUVEC)损伤模型,以温脉通及其温经益气拆方和活血通脉拆方含药血清进行干预。光镜下观察细胞形态变化,MTT法检测细胞活性;取细胞上清液,以分光光度计比色法测定乳酸脱氢酶(LDH)含量,硝酸还原酶法测定NO含量;放射免疫测定法测定内皮素(ET)含量。结果温脉通及其拆方均能使高脂损伤的HUVEC形态趋于正常,活性增强,细胞膜损伤减轻;并能使HUVEC培养上清中NO含量增加而ET含量减少。两拆方单独的作用均比全方弱。结论温脉通对高脂损伤的血管内皮细胞有保护作用,这可能是其治疗ASO的机制之一。温脉通全方对内皮细胞损伤的保护作用比其温经益气拆方和活血通脉拆方强,说明组方具有合理性。

Objective To study the protective effect of Wenmaitong （WMT） on vascular endothelial cells （VEC） from injury induced by hyperlipidemic serum to investigate the therapeutic mechanism of WMT in treating arteriosclerosis obliterans （ASO）. Methods Hyperlipidemic serum was used to establish injured model of human umbilical vascular endothelial cell （HUVEC）. Sera with WMT or its decomposed formulas, warming yang and boosting qi part formula and promoting blood circulation part formula, were applied with hyperlipidemic serum together. The morphologic changes of HUVEC were observed under the light microscope and HUVEC activity was determined by MTT method. The contents of lactate dehydrogenase （LDH）, nitrogen monoxidum （NO） and endothelin （ET） in the supernatant of HUVEC were detected by spectrophotometer, nitrate reductase method and radioimmunoassay respectively. Results WMT and its decomposed formulas made morphologic changes of HUVEC induced by hyperlipidemic serum trend to normal, increased the activity of HUVEC and lessened the injury of cell membrane. NO content in the supernatant was increased by WMT and its decomposed formulas, while ET content in the supernatant was decreased. The effects of either of two decomposed formulas were inferior to WMT. Conclusion WMT has a protective effect which may be one of its mechanisms in treating ASO. stronger than that of the decomposed formula, which to some extent. on VEC from injury due to hyperlipidemic serum, The protective effect of WMT on VEC from injury is demonstrates the rationality of combination of WMT