降脂通脉方对ApoE基因敲除小鼠肝脏基底膜HSPG的调节作用

Regulation effect of Jiangzhitongmai Formula on HSPG of liver basal membrane in mice of ApoE gene knock-out

目的观察降脂通脉方对ApoE基因敲除小鼠实验性动脉粥样硬化肝脏基底膜硫酸乙酰肝素蛋白多糖HSPG的调节作用。方法将C57/BL小鼠12只设为正常对照组,ApoE基因敲除小鼠36只随机分为3组:模型组、降脂通脉方组、舒降之阳性对照组。正常对照组、模型组予生理盐水灌胃,给药组按正常人每公斤体重10倍的量灌胃90 d后,全自动生化分析仪测定血清血脂含量,用免疫印记法测定各组肝脏基底膜硫酸乙酰肝素蛋白多糖的表达,用Quantity One 6.0软件进行光密度分析。结果降脂通脉方组、阳性对照药组与模型组比较,总胆固醇、低密度脂蛋白水平均明显降低,有极显著差异(P<0.001),治疗组间无显著性差异。降脂通脉方组、阳性对照药组肝脏基底膜HSPG表达与模型组比较明显上调,但与正常对照组比较则明显下调。结论降脂通脉方对apoE-基因敲除高脂饲料喂养所致动脉粥样硬化小鼠具有降低血清总胆固醇、低密度脂蛋白的作用,对该模型小鼠的肝脏基底膜HSPG表达有调节作用,而降脂通脉方对HSPG表达的上调,是防治动脉粥样硬化的机制之一。

Objective To observe the regulation effect of Jiangzhitongmai Formula on the expression of heparan sulafae proteoglycan （HSPG） of liver basal membrane in mice with ApoE-knock-out arthrosclerosis. Methods The normal control group （ group 1 ） included 12 C57/BL mice and other 36 mice of ApoE knock-out were randomly divided into the model group （group 2 ）, Jiangzhitongmai Formula group （group 3 ） and Simvastitin control group （group 4 ）. Group 1 and 2 were given orally normal saline and group 3 and 4 were given respectively Jiangzhitongmai Formula and Simvastitin orally in the dose higher than normal （/kg） by 10 times. After 90 days the serum content of blood lipid was determined by fully automatic biochemical analyzer, the expression of HSPG was tested by immunoblot method and the photodensity was analyzed by the software of Quantity One 6.0 in different groups. Results In group 3 and 4 the levels of total cholesterol （TC） and low-density lipoprotein-C （LDL-C） were obviously decreased with a great significant difference （P 〈 0. 001 ） compared with those in group 2.There was no significant difference between group 3 and 4. The expression of HSPG was much higher in group 3 and 4 than that in group 2 but much lower than that in group 1. Conclusion Jiangzhitongrnai Formula has an effect of decreasing TC and LDL-C levels and regulating the expression of HSPG of liver basal membrane in mice with ApoE-knock-out arthrosclerosis induced by feeding high fat food. The upregulative effect of Jiangzhitongmai Formula on the expression of HSPG is one of its mechanisms for preventing and treating arthrosclerosis.