掺锶聚磷酸钙骨组织工程支架材料与成骨细胞及内皮细胞的相容性研究

Cytocompatibility of strontium-doped calcium polyphosphate tissue engineering materials to osteoblast and endothelial cells

目的:观察掺锶聚磷酸钙材料与成骨细胞及内皮细胞相容性,为该材料作为骨组织工程支架材料最终应用于临床提供实验依据。方法:实验于2006-01/07在四川大学组织工程研究室完成。在聚磷酸钙制备过程中掺入亲骨元素锶,制备新型的不同掺锶量(0.5%,1%,5%,10%,20%,30%,40%,50%,60%,70%,80%,90%)的骨组织工程支架材料掺锶聚磷酸钙。以聚磷酸钙、羟基磷灰石及磷酸三钙为对照,通过MTT法及扫描电镜观察掺锶聚磷酸钙的成骨细胞及血管内皮细胞的相容性。结果:无论是对成骨细胞还是内皮细胞,1%掺锶聚磷酸钙材料均显示出更好的相容性:MTT法表明,与对照组及其他掺锶量的掺锶聚磷酸钙比较,1%掺锶聚磷酸钙材料拥有更高的成骨细胞增殖度;与聚磷酸钙比较,1%掺锶聚磷酸钙材料拥有更高的内皮细胞增殖度;扫描电镜表明在1%掺锶聚磷酸钙材料表面生长的成骨细胞或内皮细胞具有更好的生长状态和生物活性。结论:掺锶聚磷酸钙是一个很有前途的骨组织工程支架材料,它不仅与成骨细胞具有很好的相容性,而且能促进内皮细胞的增殖及诱导新生血管的形成,有望解决植入材料血管化的难题。

AIM： The cytocompatibility to osteoblast and endothelial cells of strontium-doped calcium polyphosphate （SCPP） is observed to offer an experimental foundation for clinical researches as scaffolds for bone tissue engineering. METHODS： The experiment was performed at the Tissue Engineering Research Laboratory of Sichuan University from January to July 2006. By introducing strontium into calcium polyphosphate （CPP）, a novel scaffold for bone tissue engineering, named SCPP, was prepared at different dosages of strontium （0.5%, 1%, 5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%）. Taking CPP, hydroxylapatite and tricalcium phosphate as controls, the cytocompatibility to osteoblast and endothelial cells of SCPP was investigated by means of 3-（4,5-dimethylthiazolyl）-2,5-diphenyltetrazolium bromide test （MTT test） and scanning electron microscope. RESULTS： The SCPP containing 1% strontium displayed better compatibility to osteoblast and endothelial cells： The result of MTr test showed that the proliferous level of osteoblast on the SCPP containing 1% strontium was higher than those of the controls and other dose strontium; The proliferation of endothelial cells on the SCPP containing 1% strontium showed a higher level compared to the CPP; The osteoblast and endothelial cells on the SCPP containing 1% strontium presented better growth and bioactivity under scanning electron microscope. CONCLUSION： SCPP may serve as a more promising scaffold for bone tissue engineering. It not only has good compatibility with osteoblast, but also induces the proliferation of endothelial cells and angiogenesis within the materials. Thus the vascularization of implanted materials is likely to be solved.