增生性瘢痕增生期与成熟期相关基因谱表达的差异

Difference in the expression of associated gene-cluster between proliferative and mature stage hypertrophic scars

目的:从基因水平探索增生性瘢痕退行性变的机制,筛选调控增生性瘢痕从过度增殖向成熟稳定期转化的退行性变相关基因。方法:实验于2003-05/2005-03在解放军第二军医大学整形外科实验室和上海联合基因集团博星基因芯片公司进行。收集8例烧伤后增生性瘢痕患者的病理标本,每张芯片以同一患者同一部位退变成熟期的瘢痕组织为实验组,增生活跃期的瘢痕组织为对照组。按一步法抽提出瘢痕组织总RNA,纯化mRNA,分别制成Cy3和Cy5荧光标记cDNA探针,与含有14112个cDNA的高密度基因微矩阵芯片杂交,通过计算机扫描生物信息学分析筛选差异表达基因。结果:在14112个基因中,8例患者退变后稳定期的增生性瘢痕组织与增殖活跃的增生性瘢痕组织之间比较存在差异表达基因共有840条,其中上调基因438条,下调基因362条,新基因102条;包括细胞凋亡基因、原癌基因和抑癌基因、细胞骨架和运动基因、细胞受体、免疫相关、细胞信号和传递蛋白、代谢蛋白、发育相关等多种基因。结论:增生性瘢痕的退行性变与多种基因的差异表达有关。

AIM： To explore the mechanism of the degradation of hypertrophic scar at gene level and screen the degrading associated genes regulating the hypertrophic from hyperplasia to mature stable stage. METHODS： The experiment was conducted in the laboratory of Department of Plastic Surgery, Second Military Medical University of Chinese PLA and BioStar Genechip Inc., Shanghai United Gene Group between May 2003 and March 2005. Samples from 8 patients with hypertrophic scar after bum were collected. The scar tissues at the mature stage from the same part and patient served as experiment group, and the tissues at proliferative stage as the control group. The total RNA of scar tissues were extracted by one step method and purified to prepare cDNA exploring needles labeled with Cy3 and Cy5, which was hybridized with high density microarrays containing 14 112 cDNA. The differentially expressed genes were screened by computer scanning bioinformatics analysis. RESULTS： 840 genes, in which 438 were overexpressed genes and 362 were downexpressed in mature stage scars compared with proliferative stage scars, were found to be differentially expressed in 14 112 genes in the samples and 102 novel genes were included. These differentially expressed genes included apoptosis-related genes, oncogenes, tumor suppression genes, cell receptor and skeleton related genes, immune related genes, signal-transduction-related genes, and development related genes. CONCLUSION： The degrading process of hypertrophic scar is related with the differentially expressed associated genes.