亚低温对脑缺血再灌注损伤后神经功能的保护作用及机制研究

A Study on Protective Effect of Mild Hypothermia on Neurobehavior in Rats Undergoing Cerebral Ischemia Reperfusion Injury

目的：验证亚低温对脑缺血后神经功能的保护作用．方法：将４６只ＳＤ大鼠随机分为对照组、缺血组和亚低温治疗组（肛温维持在３２～３３℃之间），分别在急性和慢性条件下进行．参照Ｐｕｌｓｉｎｅｌｉ和Ｂｒｉｅｒｌｅｙ的方法建立脑缺血动物模型，并观察神经行为学、病理学及Ｎａ＋－Ｋ＋－ＡＴＰ酶活性与脑组织含水量的改变．结果：与对照组和缺血组相比，亚低温能显著缓解Ｎａ＋－Ｋ＋－ＡＴＰ酶活性的降低（Ｐ＜０．０１），改善神经行为学异常并降低脑组织含水量（Ｐ＜０．０５）．结论：亚低温能明显减轻缺血后脑组织病理形态学的损害程度，对神经功能的恢复起促进作用．其机制与恢复能量供给，保护血脑屏障，减轻脑水肿等有关．

Aim: To evaluate the protective effects of mild hypothermia on neurofunction after cerebral ischemia.Methods: Forty six SD rats were divided into a control group, an ischemic group and a hypothermic group (anal temperature between 32-33℃) under acute and chronic conditions respectively by means of Pulsinelli and Brierley's animal model. Meanwhile changes of neurobehavior, pathological changes, activity of Na + K + ATPase and brain water content were observed. Results: Compared with the control and ischemic groups, mild hypothermia could significantly reduce brain water content, increase activity of Na + K + ATPase ( P <0.01) and ameliorate the behavior pattern. Conclusion: These data show that mild hypothermia can markedly reduce the pathological changes of postischemic brain structures and improve the recovery of neurofunction. These effects may be related to restoration of energy supply, prevention of brain blood barrier and amelioration of cerebral edema.