骨髓瘤细胞分化过程中转录因子Blimp-1对c—mye基因表达的调控被引量：1

Regulation of c-myc expression by Blimp-1 during the differentiation of myeloma cells

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摘要目的探讨2-甲氧基雌二醇（2-methoxyestradiol，2ME2）诱导骨髓瘤细胞分化过程中转录因子Blimp—1对c—myc基因表达的调控作用。方法用0．5μmol／L 2ME2处理骨髓瘤细胞系CZ-1和LP-1细胞72h，以诱导转录因子Blimp—1表达上调，用RT—PCR和Westernblot检测c—myc基因mRNA及蛋白的表达情况；另用0．5μmol／L2ME2＋Blimp—1反义寡核苷酸（ASODN）处理CZ—1和LP-1细胞72h，采用细胞形态学、流式细胞术检测细胞表面分化抗原CD49e的表达，ELISA法检测细胞分泌免疫球蛋白的水平，观察Blimp-1 ASODN对细胞分化作用的影响，同时采用RT—PCR和Western blot检测c—myc基因mRNA及蛋白的表达情况。结果当两种骨髓瘤细胞系采用2ME2处理72h后Blimp—1的表达均上调，c—myc基因mRNA及其蛋白的表达下调；而当Blimp—1表达受到ASODN封闭未出现上调时，细胞分化过程受到抑制，骨髓瘤细胞未出现成熟浆细胞的形态，细胞表面分化抗原CD49e的表达以及细胞分泌的免疫球蛋白水平与对照组相比，差异无统计学意义，此时c—myc基因mRNA及其蛋白的表达水平也未出现下调。结论2ME2诱导骨髓瘤细胞分化的信号转导通路中，转录因子Blimp—1抑制c—myc基因mRNA及其蛋白的表达。 Objective To investigate the repression of c-myc induced by Blimp-1 in 2-methoxyestradiol（2ME2）-mediated differentiation of multiple myeloma cells. Methods CZ-1 and LP-1 myeloma cells lines were exposed to 0.5 μmol/L of 2ME2 and 0.5 μmol/L of 2ME2 ＋ antisense oligonucleotides （ASODN） for 72 h. The effects on transcription of c-myc mRNA were studied by RT-PCR. The c-myc protein was assayed with Western blot. The changes of the cell lines in morphology, expression of surface CD49e and quantity of immunoglobin light chain secretion in the supernatant were studied. Results Incubation of the cells with 0.5 μmol/L of 2ME2 could up-regulated Blimp-1 expression and increase the expression of c-myc gene. In contrast, c-myc expression was decreased with Blimp-1 expression down-regulated by ASODN and the cell lines differentiation was arrested. Conclusion Blimp-1 could directly repress the expression of c-myc in 2ME2-mediated differentiation induction of multiple myeloma cells.

作者周霞周帆姜华侯健

机构地区第二军医大学长征医院血液科长征医院闸北分院血液肿瘤科

出处《中华血液学杂志》 CAS CSCD 北大核心 2007年第5期318-322,共5页 Chinese Journal of Hematology

关键词雌二醇多发性骨髓瘤细胞系细胞分化基因 BLIMP-1 C-MYC Estradiol Multiple myeloma Cell lines Cell differentiation Gene, Blimp-1 Gene,c-myc

分类号 R686 [医药卫生—骨科学]

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参考文献9

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