聚合酶链反应在沙眼衣原体感染患者随访中的意义

Polymerase chain reaction in detected of Patients Infected by chlamydia trachomatis after treatment.

研究聚合酶链反应（ＰＣＲ）方法在沙眼衣原体感染患者随访观察中的意义。方法对３０例患者进行治疗后随访。３０例患者在治疗前ＰＣＲ和直接免疫荧光法（ＤＦＡ）检测均阳性，其中１５例患者服用美满霉素１００ｍｇ，每日２次，连用１０日；１５例患者服用阿奇霉素１．０ｇ，一次顿服。结果停药１～２周后复查，３０例患者ＤＦＡ检测全部为阴性，３０例患者中ＰＣＲ检测９例仍阳性，２１例阴性。结论沙眼衣原体感染患者在服用抗生素后，ＰＣＲ阳性不能肯定为有活性的沙眼衣原体存在，因其死亡的ＤＮＡ片段可在体内存留１个月，只要有ＤＮＡ片段存在，ＰＣＲ检测均为阳性。

Objective To investigate the value of polymerase chain reaction (PCR) for follow up patients infected by Chlamydia trachomatis Methods Follow up specimens were collected from 30 patients. Chlamydia trachomatis positive were detected by PCR and direct flurescence assay test (DFA) in the 30 patients before therapy. 15 patients were treated with minocycline(100mg twice daily)for 10 days, and 15 patients were treated with 1.0g of azithromycine as a single oral dose.Results After 1 2 weeks of antimicrobiol therapy, all patients had negative DFA for Chlamydia trachomatis, but 9 had postitive Chlamydia trachomatis DNA as detected by PCR. Conclusions The 9 specimens were not confimed to livae viable organisms of Chlamydia trachomatis. The debris of nonviable Chlamydia trachomatis DNA was exluded from urinogenital tract at about one month.