摘要
研究聚合酶链反应(PCR)方法在沙眼衣原体感染患者随访观察中的意义。方法对30例患者进行治疗后随访。30例患者在治疗前PCR和直接免疫荧光法(DFA)检测均阳性,其中15例患者服用美满霉素100mg,每日2次,连用10日;15例患者服用阿奇霉素1.0g,一次顿服。结果停药1~2周后复查,30例患者DFA检测全部为阴性,30例患者中PCR检测9例仍阳性,21例阴性。结论沙眼衣原体感染患者在服用抗生素后,PCR阳性不能肯定为有活性的沙眼衣原体存在,因其死亡的DNA片段可在体内存留1个月,只要有DNA片段存在,PCR检测均为阳性。
Objective To investigate the value of polymerase chain reaction (PCR) for follow up patients infected by Chlamydia trachomatis Methods Follow up specimens were collected from 30 patients. Chlamydia trachomatis positive were detected by PCR and direct flurescence assay test (DFA) in the 30 patients before therapy. 15 patients were treated with minocycline(100mg twice daily)for 10 days, and 15 patients were treated with 1.0g of azithromycine as a single oral dose.Results After 1 2 weeks of antimicrobiol therapy, all patients had negative DFA for Chlamydia trachomatis, but 9 had postitive Chlamydia trachomatis DNA as detected by PCR. Conclusions The 9 specimens were not confimed to livae viable organisms of Chlamydia trachomatis. The debris of nonviable Chlamydia trachomatis DNA was exluded from urinogenital tract at about one month.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1997年第2期91-93,共3页
National Medical Journal of China
关键词
沙眼衣原体
聚合酶链反应
尿道炎
非淋菌性
Chlamydia Trachomatis Follow Up Studies Polymerase Chain Reaction