肿瘤坏死因子在大鼠原位肝移植急性排斥反应中的作用

The role of TNFα in the allograft rejection of orthotopic rat liver transplantation.

测定原位肝移植大鼠术后血清肿瘤坏死因子α（ＴＮＦα）浓度，探讨其在原位肝移植急性排斥反应中的作用。方法利用ＲＴ－ＰＣＲ检测大鼠移植肝组织内ＴＮＦαｍＲＮＡ的表达，并观察移植肝病理形态变化。结果术后３、６天异基因大鼠血清ＴＮＦα浓度都显著高于同期同基因肝移植大鼠和正常对照鼠血清ＴＮＦα浓度。在异基因原位肝移植大鼠术后第６天，肝标本中检测到ＴＮＦαｍＲＮＡ表达，而在同基因原位肝移植大鼠同期肝标本和正常大鼠肝标本中均未检测到ＴＮＦαｍＲＮＡ的表达。结论ＴＮＦα在排斥反应中发挥重要作用，术后监测ＴＮＦα浓度变化对急性排斥反应的发生有一定预测和早期诊断作用。

Objectives To measure tumor necrosis factor α (TNFα) in rats on the 3rd, 6rd days after orthotopic rat liver transplantation, and detect the expression of TNFα mRNA in the grafts. Method Reverse transcription polymerase chain reaction (RT PCR) was used. Results The TNFα serum levels on postoperative day 3, 6 were significantly higher in allograft group than in isogeneic graft group. RT PCR analysis revealed that TNFα mRNA was present in allografts, while no TNFα mRNA expression occurred in isogeneic grafts. Our findings supported that TNFα plays an important role in the pathogenesis of acute allograft rejection, and suggested that sequential determinations of TNFα after operation may be of value in the prediction and diagnosis of acute rejection.