汉防己甲素对血小板衍生生长因子促细胞增殖效应的阻断作用

Blocking action of tetrandrine on the cell proliferation induced by PDGF in human lung fibroblasts and liver Ito cell of rats

目的研究血小板衍生生长因子（ｐｌａｔｅｌｅｔ－ｄｅｒｉｖｅｄｇｒｏｗｔｈｆａｃｔｏｒ，ＰＤＧＦ）对人成纤维细胞（ＨＬＦ）、大鼠肝贮脂细胞（Ｉｔｏ细胞）增殖活性和胶原合成的影响及汉防己甲素（Ｔｅｔ）对ＰＤＧＦ促细胞增殖效应的阻断作用。方法采用胶原酶灌流、密度梯度离心方法分离大鼠肝Ｉｔｏ细胞，并以Ｈ－ＴｄＲ、Ｈ－Ｌ－脯氨酸掺入法观察细胞增殖效应。结果在１％血清存在下，ＰＤＧＦ对ＨＬＦ、Ｉｔｏ细胞具有显著的促进ＤＮＡ合成和细胞增殖的作用，并随ＰＤＧＦ浓度的升高而作用增强；对无血清培养的ＨＬＦ、Ｉｔｏ细胞并无明显的促进增殖作用，但与胰岛素样生长因子（ＩＧＦ）等联合应用时，则可促进细胞的增殖，并证实ＰＤＧＦ具有促进ＨＬＦ、Ｉｔｏ细胞胶原合成的作用。Ｔｅｔ在不抑制细胞ＤＮＡ合成的剂量范围内，即可拮抗ＰＤＧＦ的促细胞增殖及胶原合成作用。结论ＰＤＧＦ对肝纤维化相关细胞ＨＬＦ、Ｉｔｏ细胞增殖活性及胶原合成具有促进作用，Ｔｅｔ对ＰＤＧＦ促细胞增殖效应具有阻断作用。

Objective To investigate the mechanism of hepatic fiberosis,the effects of platelet derived growth factor(PDGF)on cell proliferation activity and collagen synthesis in human lung fibroblasts(HLF)and Ito cells of rat liver as well as the blocking action of tetrandrine on the cell proliferation induced by PDGF.Methods The Ito cells were separated from liver of rats by using the perfusion of collagenase and density pradient centrifugation.The cell proliferation was observed with the incorporation method of 3 H TdR and 3 H L proline.Resuits Under condition of cell culture with1%serum,PDGF could significantly enhance DNA synthesis,cell proliferation and collagen synthesis,which increased in degree with a rising of concentration of PDGF.This occurred in culture without serum,but under the same condition,if PDGF was applied together with insulin like growth factor(IGF),it enhanced cell proliferation.The result also showed that the dose of tetrandrine below the level of inhibiting cell DNA synthesis could antagonize the stimulation action of PDGF on cell proliferation and collagen synthesis.Conclusion Tetrandrine plays the blocking action in the promoting cell proliferation induced by PDGF.