缺氧诱导因子-1α及诱导型一氧化氮合酶在中耳胆脂瘤上皮中的表达及意义

Expression and Significance of HIF-1α and iNOS in human middle ear cholesteatoma epithelium

目的:研究缺氧诱导因子-1α(HIF-1α)、诱导型一氧化氮合酶(iNOS)在中耳胆脂瘤上皮中的表达及意义,探讨其在发病机制中的可能作用。方法:运用免疫组织化学SP法检测21例胆脂瘤标本及11例正常外耳道皮肤标本中HIF-1α、iNOS的表达。结果:在21例中耳胆脂瘤上皮及11例正常外耳道皮肤中,HIF-1α的表达指数分别为52.49±13.80、0.60±0.49,两者比较差异有统计学意义(P<0.01);iNOS的表达指数分别为92.05±27.84、1.15±0.84,两者比较差异有统计学意义(P<0.01);HIF-1α与iNOS的表达指数呈正相关(r=0.536,P<0.05)。结论:HIF-1α及iNOS蛋白在中耳胆脂瘤上皮中存在高度表达;缺氧及其相关因子HIF-1α、iNOS等可能在中耳胆脂瘤的发生、发展过程中起着重要促进作用。

Objective：To investigate the expression of Hypoxia inducible factor-1α（HIF-1α） and inducible Nitric Oxide Synthase （iNOS） in cholesteatoma and approach the possible role of them in the formation and development of the middle ear cholesteatoma. Method：Immunohistochemical SP method was used to examine the expression of HIF-1α and iNOS protein in 21 middle ear cholesteatoma specimens and 11 samples of normal external ear canal skin. Result：In the 21 middle ear cholesteatoma, in epithelium tissue samples and 11 external ear cannel＇s normal skin specimens, the expression index of HIF-1α was 52.49＋13.80, 0.60＋0.49, and that of iNOS was 92.05 ＋ 27.84, 1.15 ＋0.84 respectively. The HIF-1α and iNOS expression index of cholesteatoma epithelium was significantly higher than that of external ear cannel＇s normal skin （ P〈0.01）. Moreover in the cholesteatoma epithelium, linear correlat ion analysis showed that iNOS protein was positively correlated with HIF-1α protein expression. （ r =0. 536, P 〈0.05）. Conclusion： High density of HIF-1α and iNOS are found in the epithelium of cholesteatoma; Hypoxia and its relative factors HIF-1α,iNOS may play an important role in the formation and development of cholesteatoma.