肠道细菌CGC定植及其对局部细胞活性的影响

Colonization of bacterial cryptic growth cells and its influence on the local cellular activity

目的:探讨肠道细菌CGC在体内定植分布的主要位置及这种定植对局部细胞活性的影响.方法:采用头孢呋辛钠诱导CGC产生,以体内细菌定植的原位检测方法对结肠各段CGC定植情况进行检测.在建立了IBS动物模型基础上,采用高效液相色谱法对CGC定植较多的回盲部肠黏膜上皮细胞的腺苷酸能荷及ATP与腺苷酸库比值进行测定,以反映细胞活性状态.结果:正常大鼠检测未见大量细菌CGC,在模型鼠标本的连续检测结果中检出大量CGC.通过肠道细菌定植检测,细菌CGC主要定植在回盲部和升结肠段;与正常组相比,IBS模型组回盲部三个肠段的细胞线粒体ATP能荷值以及ATP与总腺苷酸库比值检测结果明显降低,相应肠段之间数值差异显著(均P<0.05).结论:细菌CGC在大鼠体内定植显优势分布,在局部定植后能引起细胞活性降低.

AIM： To explore the predominant location and the influence of bacterial cryptic growth cell （CGC） colonization on the activity of local enterocytes. METHODS： The bacterial CGC was induced by the antibiotics Cefuroxime, and the colonized status was detected by the techniques of local colonization in vivo. After the establishment of irritable bowel syndrome （IBS） model, the energy charge （EC） of adenosine triphosphate （ATP） and the ratio of ATP to the total amount of adenylic acid pool in colonic epithelial cells at the ileocecum with better colonization were determined by high performance liquid chromatography （HPLC）. RESULTS： Normal rats had just a few bacterial CGC, but a great number of bacterial CGC were detected in model rats. The bacterial CGC colonized mainly at the ileocecum and ascending colon and fewer at the other parts; the energy charge of ATP and the ratio of ATP to the total amount of adenylic acid pool in the three parts of ileocecal colon of IBS rats were significantly lower than those in the normal control groups, respectively （both P 〈 0.05）. CONCLUSION： CGC colonization causes a down-regulation of the cellular activity, which may help to explore the mechanism of IBS pathogenesis.