软脉灵对大鼠非酒精性脂肪肝的预防作用

Preventive effect of Ruanmailing on non-alcoholic fatty liver disease in rats

目的：观察软脉灵对非酒精性脂肪性肝病大鼠模型的预防作用.方法：采用高脂膳食喂养方式建立大鼠非酒精性脂肪性肝病模型.♂SD大鼠90只,随机分为模型组、药物组与空白对照组,每组30只.模型组应用高脂饲料喂养,药物组在高脂饲料喂养同时应用软脉灵灌胃,空白对照组应用基础饲料喂养.每组均于8,12及16 wk时各处死10只,观察肝脏指数,血清与肝组织生化指标及肝组织病理改变.结果：16 wk时与模型组相比较,药物组肝脏指数显著降低（F=51.626,P=0.000）;血清胆固醇（CHOL）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-c）,丙氨酸转氨酶（ALT）及谷草转氨酶（AST）水平明显降低（2.27±0.38 mmol/Lvs 3.09±0.45 mmol/L;0.83±0.13 mmol/L vs1.03±0.18 mmol/L;0.41±0.06 mmol/L vs1.09±0.27 mmol/L;52.0±6.50 U/L vs 79.0±4.72 U/L;182.9±26.43 U/L vs 326.5±28.21U/L;均P＜0.01）,但血清高密度脂蛋白胆固醇（HDL-c）水平提高（0.76±0.17 mmol/L vs0.55±0.11 mmol/L,P＜0.01）;肝组织丙二醛（MDA）水平降低明显（167.2±14.00 mmol/g vs 263.6±26.84 mmol/g,P＜0.01）,超氧化物歧化酶（SOD）活力增加显著（9.95±0.33 U/g vs 4.36±0.46 U/g,P＜0.01）,肝组织脂肪变性程度（x^2=1 9.828-20.470,均P=0.000）和炎症活动度（F=10.170,P=0.000）明显减轻.结论：软脉灵具有预防大鼠非酒精性脂肪性肝病的作用.

AIM： To investigate the effect of Ruanmailing in preventing nonalcoholic fatty liver disease （NAFLD） in rats. METHODS： Rat models of NAFLD were established by high-fat diet. A total of 90 male Sprague Dawley rats were randomly divided into group A, B and C, fed with high-fat diet, high-fat diet plus Ruanmailing （ig）, and normal basic food. Ten rats of each group were sacrificed at the 8^th, 12^th and 16^th week, and meanwhile, the liver index, biochemical indicators in serum and liver tissues and the hepatic pathological changes were observed. RESULTS： At the 16^th week in group B, the liver index was decreased significantly in comparison with that in group A （F = 51.626, P = 0.000）; the serum levels of cholesterol （CHOL）,triglyceride （TG）, low-density lipoprotein cholesterol （LDL-C）, alanine aminotransferase （ALT） and aspartate aminotransferase （AST） were reduced markedly （2.27 ± 0.38 mmol/L vs 3.09 ± 0.45 mmol/L; 0.83 ± 0.13 mmol/L vs 1.03 ± 0.18 mmol/L; 0.41 ± 0.06 mmol/L vs 1.09 ± 0.27 mmol/L; 52.0 ± 6.50 U/L vs 79.0 ± 4.72 U/L; 182.9 ± 26.43 U/L vs 326.5 ± 28.21 U/L; all P 〈 0.01）, while the level of high-density lipoprotein cholesteror （HDL-C） was elevated （0.76 ± 0.17 mmol/L vs 0.55 ± 0.11 mmol/L, P 〈 0.01）; the tissue content of malondialdehyde （MDA） was decreased evidently （167.2 ± 14.00 mmol/g vs 263.6 ± 26.84 mmol/g, P 〈 0.01）, while the activity of superoxide dismutase （SOD） was enhanced notably （9.95 ± 0.33 U/g vs 4.36 ± 0.46 U/g, P 〈 0.01）; the degree of fatty degeneration （x^2 = 19.828-20.470, all P = 0.000） and inflammation （F =10.170, P = 0.000） were relieved dramatically. CONCLUSION： Ruanmailing is effective in preventing NAFLD in rats.