卡巴胆碱对肠上皮细胞氧化损伤的保护作用

Protective effects of carbachol on intestinal epithelial cells against oxidative injury

目的:探讨卡巴胆碱对大鼠肠上皮细胞(intes- tinal epiIhelia cell,IEC)过氧化损伤的影响.方法:将H_2O_2加入IEC培养液中制成IEC过氧化损伤模型.实验设对照组、H_2O_2组(2.5 mmol/L)、卡巴胆碱组(卡巴胆碱100μmol/L).用四甲基偶氮唑盐(MTT)实验检测IEC的存活率,测定培养液中测乳酸脱氢酶(LDH)和细胞中丙二醛(MDA)含量.结果:与对照组相比,H_2O_2组LDH(7.40±2.10 vs 0.81±0.12,P<0.01)、MDA水平显著增高(P<0.0 1),细胞活力明显降低(37.25%±0.80%vs 100%±0.13%,P<0.01).卡巴胆碱组与H_2O_2组相比,LDH漏出MDA形成明显减少(4.64±1.31 vs 7.40±2.10,P<0.01),细胞的细胞活力显著增加(78.70%±2.80%vs 37.25%±0.80%,P<0.01).结论:卡巴胆碱对大鼠IEC过氧化损伤具有保护作用.

AIM： To study the effects of carbachol on the H2O2-oxidative injury of intestinal epithelial cells （IEC）. METHODS： IEC were cultured with the presence of H2O2 in vitro to induce the oxidative injury. IEC were divided into 3 groups： control group, H2O2 （2.5 mmol/L） group and carbachol （100μmol/L） group. The viability of IEC was measured by MTT assay. The level of lactate dehydrogenase （LDH） in culture media and the malondialdehyde （MDA） content in IEC were measured. RESULTS： In compared with those in control group, LDH level （7.40 ± 2.10 vs 0.81 ± 0.12, P 〈 0.01） and MDA content in H2O2 group were sig-nificantly elevated, and the viability of IEC was obviously decreased （37.25% ± 0.80% vs 100% ± 0.13%, P 〈 0.01）. However, in carbachol group, LDH level （4.64 ± 1.31 vs 7.40 ± 2.10, P 〈 0.01） and MDA content were lower while IEC viability was obviously higher than those in H2O2 group （78.70% ± 2.80% vs 37.25% ± 0.80%, P 〈 0.01）. CONCLUSION： Carbachol can protect rat IEC from the oxidative injury.