胃癌Runx3基因的甲基化

Methylation status of Runx3 gene in human gastric cancer

目的:探讨Runx3基因甲基化与胃癌的关系方法:采用MSP法检测38例配对胃癌组织、癌旁正常组织和转移淋巴结中Runx3基因甲基化的情况.结果:73.7%的胃癌组织中存在Runx3基因异常甲基化,而相应的癌旁正常组织和转移淋巴结中该基因的甲基化率分别为21.1%和65.8%.癌组织和转移淋巴结中Runx3基因甲基化的发生率显著高于癌旁正常组织(P<0.05).胃癌组织中,该基因甲基化与肿瘤大小显著相关(P =0.021),但与肿瘤大体类型、分化程度、浸润深度及生长方式等临床病理特征无关.结论:Runx3基因异常甲基化是胃癌发生、发展过程中的频繁事件,通过检测胃黏膜组织及淋巴结中该基因的甲基化情况,可能会对胃癌的早期诊断及判断淋巴结的微转移提供一定的参考价值.

AIM： To investigate the relationship between the methylation status of Runx3 gene and human primary gastric cancer. METHODS： The methylation status of Runx3 gene in 38 gastric carcinoma specimens, the corresponding normal gastric mucosa and lymph nodes was detected by methylation-specific polymerase chain reaction （MS-PCR）. RESULTS： Aberrant methylation of Runx3 gene was detected in 73.7% of gastric cancer specimens, 65.8% of lymph nodes and 21.1% of the corresponding normal mucosa, and there were significant differences between the former two and the latter. Runx3 methylation was significantly correlated with the tumor size （P = 0.021）, but not with the general types, differentiation degree, invasion depth and growth style of gastric cancer. CONCLUSION： Aberrant methylation of Runx3 gene is a common event in the occurrence and progression of gastric cancer, which may provide useful information for the early diagnosis and assessment of lymph node metastasis.