摘要
目的:检测血红素氧合酶-1(HO-1)在反流性食管炎(RE)患者食管黏膜中的表达,以探讨气体信使分子一氧化碳(CO)在RE发病机制中的作用.方法:采用免疫组化SP法结合血红素氧合酶(HO-1)的免疫反应阳性产物的平均光密度图像分析法检测77例RE患者(分4级组)及20例对照组的食管黏膜HO-1蛋白的表达强度.结果:HO-1主要在食管黏膜上皮细胞中表达,RE患者食管黏膜中表达明显强于对照组(0.2334±0.0511 vs 0.1776±0.0164,P<0.01).结论:HO-1在RE患者中的食管黏膜中的表达异常,表明CO在胃肠动力性疾病如RE的发病机制中可能起重要作用.
AIM： To detect the expression of heme oxygenase-1 （HO-1） in human esophageal mucosa with reflux esophagitis （RE）, and to find out the role of gas messenger molecule carbon monoxide （CO） in the pathogenesis of RE.
METHODS： The distributions of HO-1 protein in the esophageal tissues of 77 RE cases and 20 normal controls were detected by immunohistochemistry （SP method）.
RESULTS： HO-1 was expressed mainly in the esophageal mucosal epithelium, The expression of HO-1 in RE patients was significantly higher than that in the normal controls （0.2334 ± 0.0511 vs 0.1776 ± 0.0164, P 〈 0.01）.
CONCLUSION： Abnormal expression of HO-1 exists in RE, suggesting that CO may play an important role in the pathogenesis of gastrointestinal motility disorders such as RE.
出处
《世界华人消化杂志》
CAS
北大核心
2007年第11期1310-1313,共4页
World Chinese Journal of Digestology
