硫酸钡吸光比浊法测定大蒜中大蒜素含量

Determination of the Garlicin Content in Garlics by Barium Sulfate Turbidimetry

用浓硝酸将大蒜中有机硫化物定量氧化成硫酸根,在聚乙烯醇介质中与钡离子反应生成稳定的硫酸钡悬浊液,吸光比浊法测定其硫酸根含量,从而换算出大蒜素含量。建立了吸光比浊法测定大蒜中大蒜素含量的新方法,该法不需大蒜素标准样品,简单、快速、实用,结果与定硫法(重量法)基本一致。

An analytic method was developed for the determination of garlicin in garlic. The sample was oxidized with concentrated nitric acid, converting sulfur into sulfate, precipitated with barium chloride using polyvinyl alcohol as the stabilizer. The turbidity of barium sulfate was determined with a spectroscope,and then garlicin content was calculated. This method is simple,fast, accurate,and without the need of standard sample.