一种新的磺酸吲哚菁染料的荧光光谱分析及其应用研究

Spectrofluorometric Analysis of a New Sulfonic Indole Cyanine Dye and Its Application to Protein

研究了一种新型磺酸吲哚菁染料的荧光光谱并应用于蛋白质分析。结果表明,随着染料浓度增大,染料发生J聚合,荧光峰从564nm红移至605nm。当染料处于较低浓度时,离子强度影响不明显,而在较高浓度,J聚合增强,荧光增强变化明显;乙醇有机试剂显著增强染料荧光,并发生红移。应用于蛋白质分析,染料荧光强度随牛血清蛋白BSA或人血清蛋白HSA的增加而显著增强,在最优条件下,染料荧光强度与蛋白质浓度成良好线性关系,BSA线性范围为0.20—6.00μg/mL,检出限为0.08μg/mL;HSA线性范围为0.20—6.00μg/mL,检出限为0.1μg/mL。

A new Sulfonic Indole cyanine dye shows a fluorometric spectrum with maximum emission wavelength at 564nm, which moves to 605nm as cyanine dye polymerized at some high concentration. Effect of ion intensity is not notable in the dilute solution. The addition of ethanol results in the enhancement of the fluorescence with a red shift. The titled dye became a sensitive fluorescence probe for albumin bovine （BSA） or albumin from human serum （HSA）. Under the optimum condition,the fluorescence intensity was in a good linearity with album, and then calibration curves were obtained in the range of 0. 20--6. 00μg/mL for BSA or HAS with detection limits of 0.08μ/mL and 0.1μ/mL, respectively.