辣椒素受体在肠易激惹综合征内脏痛觉过敏中的作用

The Role of Vanilloid Receptor 1 in Visceral Hyperalgesia in Irritable Bowel Syndrome

目的研究辣椒素受体(VR1)在肠易激综合征(IBS)内脏痛觉过敏中的作用。方法新生雄性SD大鼠21只分为痛觉过敏阳性对照组(P组)、阴性对照组(N组)、去辣椒素受体神经元组(D组),出生第2天D组背部皮下注射辣椒素50mg/kg。出生第8天起P组和D组给予结肠内充气刺激每天一次,连续2周,7周时给予结直肠扩张(CRD)刺激,进行腹部收缩反射(AWR)评分和腹直肌肌电记录。结果D组AWR评分低及腹直肌放电活动少(P<0.01,与P组相比)。结论辣椒素受体参与了发育期幼鼠肠激惹致IBS内脏痛觉过敏。

Objective To evaluate the role of vanilloid receptor 1 in visceral hyperalgesia in adult rats induced by colon irritation during postnatal development. Method SD Rats were divided into irritable bowel syndrome group （Group P）,negative control group （Group N）,and VRI positive neurons depriving group （Group D）. Rats in group D received capsaicin （50 mg/kg, subcutaneously） on day-2 after birth. And the rats in group P and D were given colon irritation once a day for two weeks from day-8 after birth. Behavioral test of abdominal withdraw reflex （AWR） and electrophysiological recording of abdominal rectus abdominis electromyogram （EMG） were employed after colorectal distention （CRD） on day-49 after birth. Result The AWR scores and EMG counts were lower in group D （P 〈 0.01, versus group P）. Conclusion Vanilloid receptor 1 plays a role in visceral hyperalgesia in adult rats induced by colon irritation during postnatal development.