中医药对各型慢性阻塞性肺疾病大鼠核因子κB和γ-谷氨酰半胱氨酸合酶表达的影响

Effects of TCM Treatment According to Syndrome Differentiation on Expressions of Nuclear Factor-κB and γ-Glutamylcysteine Synthetase in Rats with Chronic Obstructive Pulmonary Disease of Various Syndrome Types

目的通过观察慢性阻塞性肺疾病(COPD)大鼠核因子κB和γ-谷氨酰半胱氨酸合酶(γ-GCS)水平的变化,探讨中医药治疗COPD的机理。方法采用改良烟熏加气管滴加脂多糖方法复制COPD模型,以免疫组化法检测核因子κB和γ-GCS水平。二次造模后,各治疗组分别灌服相应证型的中药合剂,连续14天。寒痰蕴肺治疗组灌服小青龙汤煎剂,痰热阻肺治疗组大鼠灌服麻杏石甘汤煎剂,肺气虚治疗组灌服玉屏风散煎剂,脾气虚治疗组灌服六君子汤煎剂,肾气虚治疗组灌服人参蛤蚧散煎剂,模型组大鼠每天给与2mL生理盐水灌胃。空白对照组不做任何干预。结果与空白对照组相比,各模型组γ-GCS、NF-κB在支气管和肺泡上皮等都为高表达;经过中药治疗后,阳性表达率有明显降低,其差异均有显著性(P<0·05)。结论通过辨证论治各中药方剂能纠正COPD大鼠的氧化/抗氧化失衡,减少炎性反应,从而有效治疗COPD。

Objective To explore the mechanism of traditional Chinese medicine （TCM） treatment according to syndrome differentiation in treating chronic obstructive pulmonary disease （COPD） by observing the changes of nuclear factor-κB （NF-κB） and γ-glutamylcysteine synthetase （γ-GCS） expression levels in rats. Methods COPD model was established by modified method of combining fumigation and lipopolysaccharide （LPS） intra-tracheal dripping. Model rats were treated respectively for succesive 14 days according to their syndrome, that is, Xiaoqinglong Decoction to the rats of cold-phlegm accumulation in Fei, Maxing Shigan Decoction to those of heat-phlegm accumulation in Fei, Yupingfeng Decoction to those of Fei-qi deficiency, Liujunzi Decoction to those of Pi-qi deficiency, Renshen Gejie Decoction to those of Shen qi-deficiency, Besides, modcl rats in the model control group received 2mL normal saline daily, and no intervention was applied in the normal control group. The expression of γ-GCS and NF-κB was detected by immunochemistry before and after treatment. Results Compared with that in the normal rats, the expressions of γ-GCS and NF-κB in bronchial and alveolar epithelium of COPD rats before treatment wcre significantly higher, but the positive expression rates were lowered after treatment significantly （P 〈 0.05）. Conclusion TCM treatment according to syndrome dif ferentiation could rectify imbalance of oxidation/anti-oxidation and alleviate inflammatory reaction in COPD rats, thus to treat COPD effectively.