运动训练结合电针治疗对脑缺血再灌注大鼠海马齿状回区巢蛋白表达的影响

Effects of movement exercise combined with electroacupuncture on expression of Nestin in hippocampus den-tate gyrus after cerebral ischemia-reperfusion

目的探讨运动训练结合电针治疗对脑缺血再灌注大鼠海马齿状回区巢蛋白表达的影响。方法54只Wistar大鼠随机分为造模对照组(A组)、运动训练组(B组)和运动训练结合电针治疗组(C组),采用大鼠局灶性脑缺血再灌注模型,大脑中动脉阻塞1h,再灌注7,14和21 d,应用免疫组织化学方法分别检测各组大鼠缺血侧和对侧海马齿状回区巢蛋白的表达情况。结果3组大鼠均表现为7 d时的海马区阳性细胞最多,而且在各时间点的缺血侧海马DG区的巢蛋白阳性细胞数均明显多于对侧DG区(P<0.01)。7,14和21 d时,C组和B组大鼠缺血侧海马区巢蛋白阳性细胞较A组明显增多,差异有统计学意义(P<0.01),7 d和14 d时,C组大鼠缺血侧海马区巢蛋白阳性细胞较B组亦明显增多(P<0.01)。结论脑缺血再灌注大鼠海马区巢蛋白阳性细胞的增多存在时间规律及原位增殖特性,运动训练和电针治疗可显著增加巢蛋白阳性表达的数量。

To observe the effect of movement exercise combined with electroacupuncture on the expression of Nestin in the hippocampus dentate gyrus （DG） after cerebral ischemia-reperfusion. Methods Fiftyfour Wistar rats were used and randomly divided into a control group （ Group A） , an exercise training group （ Group B）, and an exercise training combined with electroacupuncture group （Group C）. The middle cerebral arteries （MCA） of all the rats were occluded for 1 h, followed by reperfusion for 7, 14 and 21 days. Immunohistochemistry method was used to detect the expression of Nestin in the hippocampus dentate gyrus. Results The number of Nestin-positive cells peaked in DG in all groups on the 7th day after cerebral ischemia-reperfusion. The number of Nestln-positive cells in DG ipsilateral to the ischemia-reperfusion lesion were significantly more than those in the opposite side at various time points （ P 〈0.01 ）. Significant increase of Nestin expression were detected in rats of Group B and Group C at various time points after cerebral ischemia-reperfusion when compared with that of Group A （ P 〈 0.01 ）. It was also found that the number of Nestin-positive cells of Group C were significantly more than those of Group B on the 7th and 14th days after cerebral ischemia-reperfusion （P 〈0.01 ）. Conclusion The increase of Nestin-positive cells in the DG was time-dependent and characterized by in situ proliferation after cerebral ischemia- reperfusion. Exercise training and electroacupuncture can significantly promote the expression of Nestin.