超短波治疗对早期激素性股骨头缺血性坏死兔Ⅰ型胶原蛋白的影响

The effect of ultrashort wave diathermy on expression of collagen type I in early stage of hormon-induced avascular necrosis of the femoral head

目的研究超短波治疗对早期激素性股骨头缺血坏死兔Ⅰ型胶原蛋白的影响,并探讨其作用机制。方法选择健康雄性新西兰兔40只,分为正常组10只和实验组30只。实验组采用马血清与激素复制兔激素性股骨头缺血性坏死模型后,选出20只股骨头坏死处于临床分期Ⅰ期的兔,随机分为造模组和超短波组,每组10只。超短波组给予超短波治疗,其它2组不予干预。于实验第12周末,采用免疫组织化学法检测兔股骨头组织Ⅰ型胶原蛋白的表达。结果造模组Ⅰ型胶原染色强度较正常组低,超短波组染色强度较造模组高,差异有统计学意义(P<0.05)。结论激素可抑制Ⅰ型胶原蛋白的分泌,而超短波治疗可减轻股骨头坏死程度,可能延缓或逆转股骨头坏死的进展。

Objective To observe the effect of ultrashort wave diathermy on collagen type I expression in early stage of hormon-induced avascular necrosis of the femoral head （ANFH） in rabbit. Methods A total of 40 New Zealand white rabbits were randomly divided into two groups： a control group （n = 10） and a treatment group （n = 30）. All the animals in the treatment group were injected with horse serum and methylprednisolone to establish the ANFH model, and then divided into 2 subgroups： a model group and a ultrashort wave diathermy group, which were treated accordingly. After 12 weeks of treatment, all the animals were sacrificed and collagen type I expression in the femoral head was observed. Results It was shown that the expression of the collagen type I was significantly lower in the model animals than that in the controls as indicated by the stronger immunohistochemistry staining for rabbit collagen type, while that of the ultrashort wave diathermy group was significant higher than the control group （ P 〈 0.05）. Conclusion Ultrashort wave diathermy can delay or even reverse the ANFH, which might be mediated by promoting the expression of collagen type I .