HPLC法测定穿心莲注射液中脱水穿心莲内酯的含量

Determination the Content of DehydroandrographoUde in Chuanxinlian Injection by HPLC

目的:建立HPLC法测定穿心莲注射液中脱水穿心莲内酯的含量。方法:色谱柱:Hypersil ODS2 C_(18)柱(250mm×4.6mm,5μm);流动相:甲醇-水(55:45);流速1.0ml·min^(-1),检测波长250nm。结果:脱水穿心莲内酯在0.18～0.90μg的范围内,与峰面积呈良好的线性关系(r=1.000 0)。回收率为99.5%,RSD=2.0%。结论:本法快速、准确、简便。

Objective ： To establish a HPLC method for determination the content of dehydroandrographolide in Chuanxinlian injection. Method. The Hypersil ODS2 C18 column（250 mm ×4.6 mm,5 μm） was used, the methanol-water （55： 45） as mobile phase, the flow rate was 1.0 ml. min^-1 , the detection wavelength was 250 nm. Result： The linear range of dehydroandrographolide was 0.18 - 0.90 μg（ r = 1. 000 0）, The average recovery was 99： 5% with RSD 2.0%. Conclusion： The method is simple, rapid and accurate.