HPLC法测定生血颗粒中补骨脂素、异补骨脂素的含量

Determiration of Psoralen and Isopsoralen in Hematogenesis Granules

目的:建立HPLC法测定生血颗粒中补骨脂素和异补骨素的含量。方法:色谱柱为SB-C_(18)柱(250mm×4.6mm,10μm);柱温:40℃;流动相为甲醇-水(50:50);流速为1.0ml·min^(-1);检测波长为246nm。结果:补骨脂素在1.135～45.400μg·ml^(-1)范围内、异补骨脂素在1.035～41.400μg·ml^(-1)范围内呈良好的线性关系(相关系数分别为r=0.999 9和r=1.000 0),平均回收率分别为97.05%和96.36%(RSD分别为0.78%和0.86%,n=6)。结论:所建立的方法稳定性可靠、灵敏,结果准确,可用于生血颗粒的质量控制。

Objective ： To establish an HPLC method for the determiration of psoralen and isopsoralen in hematogenesis granules. Method： SB-C18 column （250 mm × 4.6 mm ,5 μm） was used;the column temperature was maintained at 40 ℃ ;The mobile phase was methanol-water（50： 50） ; The detection wavelength was at 246 nm. Result： The concentration of psoralen showed a good linear relationship within the range of from 1. 135 μg.ml^-1 to 45.400 μg.ml^-1 （r =0.999 9） ,the recovery was 97.05% ,RSD was 0.78%. the within concentration of isopsoralen showed a good linear relationship within the range of from 1. 035 μg·ml^-1 to 41. 400 μg·ml^-1 （r = 1. 000 0）, the recovery was 96.36% ,RSD was 0.86%. Conclusion： The method is reliable stability, sensitivity and exact outcome, and therefore can be used in quality control of hematogenesis granules.