固相萃取GC/MS法分析白酒中的香气成分

Analysis of the Volatile Compounds from Chinese Wine by Solid Phase Extraction and GC/MS

利用OASIS HLB提取白酒中的醇、酯、醛等中性成分,用5%氨水清洗小柱,氮气吹干后用二氯甲烷洗脱,得到挥发性成分。清洗液和穿透液合并,经浓氨水中和后,再用MAX固相萃取柱提取其中的有机酸,用含有0.5%盐酸的乙腈洗脱得到有机酸。富集后的样品用GC/MS分离、鉴定,从中性成分中鉴定出50种成分,其中包括7种有机酸。白酒中的有机酸和其他中性成分经过两种固相萃取柱得到了较好的富集和分离。

OASIS HLB SPE was used to extract the neutral compounds from Chinese wine, such as alcohols, esters and aldehydes etc. After washed by 5% ammonia, the HLB SPE was dried by N2, and then eluted by CH2Cl2, the volatile compounds were obtained. The filtrate was neutralized by ammonia and loaded in the MAX SPE, the organic acids were eluted by 0. 5 % HCl in ACN. The volatiles and organic acids were separated and identified by GC/MS. 50 compounds are identified in the neutral, including 7 organic acids in the eluent from MAX. The organic acids and other neutral compounds in the wine are separated well by these two SPE.