辛夷挥发油不同提取方法的研究

Volatile Oil Obtained from Yulan Magnolia Flower Bud with Different Methods

采用超临界CO2萃取、微波萃取、微波提取、超声波提取、回流提取和水蒸气蒸馏6种方法提取辛夷挥发油,用正交实验法研究各种方法的最佳提取条件,并利用GC/MS分析了不同方法提取辛夷挥发油的化学成分。结果显示6种提取方法的收率分别是4.38%(超临界CO2萃取)、3.10%(微波提取)、2.90%(微波萃取)、2.80%(超声提取)、2.60%(回流提取)、2.28%(水蒸气蒸馏),共确认出52种化学成分,同时确定了各组分的百分含量,6种方法共有的主要成分为桉油精、樟脑、α,α,4-三甲基-3-环己烯基-1-甲醇、石竹烯、s-(E,E)-1-甲基-5-亚甲基-8-(甲基乙基)-1,6-环癸二烯。

The paper reduced that the volatile oils was obtained from Yulan Magnolia Flower Bud with SFE, microwave, ultrasonic distillation, regurgitation distillation and steam distillation. The optimum condition was determined by the orthogonal experiments. The yields of volatile oils extracted with six methods are 4.38%（SFE）, 3.10%（microwave distillation）, 2.90% （microwave extraction）, 2.80% （ultrasonic distillation）, 2.60% （regurgitation distillation）, 2.28 % （steam distillation）. And 52 chemical components and their rela- tive contents were identified by GC/MS method. The common main components of six methods were camphor, 3,7-dimethyl-1,6-octadien-3-ol, caryophyllene, a,a,4-trimethyl-3- cyclohexene-1-methanol and [s- （E,E）]-1-methyl-5-methylene-8-（1-methylethyl）-1, 6-cyclodecadiene. This offers us scientific base for researching active components and resource using of Yulan Magnolia Flower Bud.