非病毒载体聚乙烯亚胺介导DNA转染的研究

Optimized Polyethylenimine as a Non-viral Vector for DNA Transfection

目的研究新型非病毒类载体聚乙烯亚胺(PEI)的最适转染条件。方法分析各种因素对25 ku线性PEI转染效率的影响,优化实验条件。结果PEI与DNA的质量比≥2能保证DNA与PEI完全结合,最佳混合时间为10 min,但血清的存在将降低其结合效率。在一定范围内提高PEI与DNA的质量比有利于提高转染效率。293T细胞最适转染密度为培养面积的80%。结论确定了PEI转染细胞的最优条件。

Objective The purpose of this study is to investigate the optimal conditions for DNA transfection with polyethylenimine （PEI）. Methods The influences of various factors on the transfection efficiency of 25 kDa linear PEI were investigated to get optimal conditions. Results PEI could fully condense DNA with a PEI/DNA ratio ≥ 2, and the optimal mixing time was 10 minutes. But the presence of serum significantly lowered the efficiency of PEI. The increasing PEI/DNA ratio could raise transfection efficiency. The optimal density of 293T cells for transfection was 80%. Conclusion The factors affecting the transfection efficiency of PEI were studied, and the optimal conditions were identified.