系统性红斑狼疮患者外周血B淋巴细胞PD-L1的表达

Expression of PD-L1 on Peripheral Blood B Lymphocytes of Patients with Systemic Lupus Erythematosus

目的探讨系统性红斑狼疮(systemic lupus erythematosus,SLE)患者外周血B淋巴细胞PD-L1的表达及其在SLE发病中的意义。方法应用免疫荧光标记和流式细胞仪技术检测SLE患者外周血B淋巴细胞上PD-L1的表达水平。结果活动期SLE患者外周血B淋巴细胞CD19^+、CD19^+/PD-L1^+的表达水平明显高于SLE非活动期患者(P<0.05)和正常对照组(P<0.05)。结论活动期SLE患者B淋巴细胞的PD-L1表达异常增加,其可能在SLE发病机制中起重要作用。

Objective In order to investigate the implication of the expression of PD-L1 in peripheral blood B lymphocytes in patients with systemic lupus erythematosus （SLE）. Methods The expression of the PD-L1 on peripheral blood B lymphocytes was examined in 15 patients with active SLE, 10 patients with inactive SLE and 10 healthy controls by the technique of the immunofluorescence and the flow cytometry. Results The expression of CD19^＋, CD19^＋ PD-L1^＋ on peripheral blood B lymphocytes of the patients with active SLE was higher than those in patients with inactive SLE （P 〈0. 05） and healthy controls （P 〈0. 05）. Conclusion The expression of PD-L1 on peripheral B lymphoeytes of patients with active SLE was significanty increased, suggesting that the PD-L1 might play an important role in the pathogenesis of SLE.