白背三七地上部分降血糖作用研究

Study on Effect of the Above-ground Organs of Gynura divaricata on Reducing Blood Sugar Content

在研究白背三七地上部分化学成分的同时,对其药理作用也进行了研究.白背三七鲜样水提物3.3,6.6 mL/kg(相当于生药0.2,0.4 g/kg)及95%乙醇提取物0.75,1.50 mL/kg(相当于生药0.2,0.4 g/kg)对正常小鼠的血糖有显著的降低作用,其中1.50 mL/kg 95%乙醇提取物的降血糖效果优于格列本脲;白背三七乙酸乙酯提取物40 g(生药)/kg,正丁醇提取物4 g(生药)/kg均可明显降低正常小鼠的血糖,且乙酸乙酯提取物40 g(生药)/kg能明显降低四氧嘧啶糖尿病小鼠的血糖;白背三七总生物碱(32,16,8 mg/kg)、总黄酮(8,4,2 mg/kg)及单体化合物IV[3-O-β-D-吡喃葡萄糖(6→1)-α-L-鼠李糖山奈素](8,2 mg/kg)对正常及四氧嘧啶糖尿病模型小鼠血糖水平均有降低作用,该作用对四氧嘧啶糖尿病模型小鼠更为显著,其中对四氧嘧啶糖尿病模型小鼠血糖水平的降低作用从末次给药后0.5 h时即已显现,一直持续到末次给药后6 h,说明这些提取物和单体化合物的降血糖作用不但迅速,而且持久.

The medicinal effect of the above - ground organs of Gynura divaricata was studied. It was showed that both the water extract （ equal to 0. 2 g/kg, 0.4 g/kg crude medicine） and 95% ethanol extract （ equal to 0. 2 g/kg and 0.4 g/kg crude medicine） of the fresh G. divaricata could significantly reduce blood sugar content of the healthy mice. The dosage of 0.4 g/kg crude medicine extracted by 95% ethanol had better effect on lowering the blood sugar content than 50 mg/kg glyburide. It was also indicated by experiments that both 40 g/kg crude medicine of ethyl acetate extract and 4 g/kg crude medicine of n - butanol extract of the dried Gy-nura divaricata plants had remarkable effect on lowering blood sugar content of normal mice, and 40 g/kg crude medicine of ethyl acetate extract could also remarkably lower blood sugar content of alloan - diabetic mice. The dosage levels of 32 mg/kg, 16 mg/kg and 8 mg/kg of total alkaloid, 8 mg/kg, 4 mg/kg and 2 mg/kg of total flavone, arid 8 mg/kg,2 mg/kg of monomer compound Ⅳ （3 - 0 - β- D - （6→1）glucopyranosyl -α - L - rhamnosyl kaemferol） extracted from G. divaricata demonstrated evident lowering effect on blood sugar content of both normal and alloan - diabetic mice. The effect was more significant to the alloan - diabetic mice, which started to effect at 0.5 h after having taken the extracts and lasted till 6. 0 h to the alloan - diabetic mice, indicating that these extracts and monomer compound could work fast and have long - term effect on lowering the blood sugar content of the ill mice.