傅里叶变换中红外光纤光谱法用于腮腺肿瘤的检测

Detecting Tumor of Parotid Gland Using FT-mid-IR Fiber Optics Spectroscopy

采用红外光谱仪与中红外光纤、衰减全反射(ATR)探头联用对40例腮腺肿瘤患者的腺体体表皮肤进行了测定,并以病人术后肿瘤标本病理观察结果为判断标准,对良性及恶性肿瘤光谱进行了判别,发现光谱法判别结果与病理诊断结果基本一致.实验结果表明,正常腮腺、良性和恶性腮腺肿瘤体表皮肤的FTIR光谱中存在显著的差异,用相关吸收峰的峰形、峰位和峰强可以表征这些差异.研究进一步发现,对于不同类型的恶性肿瘤,其体表组织的红外光谱主要差别在于1500～1000cm-1区域内峰形及峰位的改变.根据这一区域的差异可以将恶性肿瘤分为两大类:一类恶性肿瘤具有上述的光谱特征(如腺泡细胞癌、嗜酸细胞腺癌和腺样囊性癌等),另一类恶性肿瘤因为类型的不同在1500～1000cm-1区域内峰形及峰位发生了很大的变化,不同于上述的光谱特征.

The modified WQF-500 FTIR spectrometer from the Second Optical Instrumental Factory and an accessory （SpecTech） of mid-IR fiber optics with an ATR probe were used to detect the skin covering a parotid gland using. In this investigation, forty patients with tumor of salivary gland were performed with FTIR fiber optics measurement. The differential diagnosis from the obtained FTIR spectra of benign and malignant tumors of salivary gland was compared with pathological diagnosis, which is considered as the standard. Good agreements were found between the results of pathological diagnosis and those of spectral detection. The results show that the difference lied in the obtained FTIR spectra of the skin covering benign and malignant tumors of salivary gland and normal parotid gland. And the difference can be characterized by the band width, peak position and peak intensity. Based on the difference of the bands in the range of 1500-1000 cm^-1, malignant tumor can be classified into two types. The first type of tumor（ for example acinic cell carcinoma, oxyphilic cell adenocarcinoma, adenoid cystic carcinoma and so on） possesses the above spectra feature. Along with the variation of species （ for example take place in spectra of the band width, peak malignant lymphoma, lympho-epithelial carcinoma and so on）, great changes position and relative intensity of the bands between 1500 and 1000 cm^-1 in other kind of malignant tumor. So the FT-mid-IR spectroscopy is a promising technique which will become a non-invasive, quick, accurate and convenient method to diagnose malignant and benign tumor of salivary gland early.