沙苑子提取物降脂作用实验研究

Experimental study on lipid-lowering effect of extracts from Semen Astragali Complanati

目的观察沙苑子提取液对实验性高脂血症大鼠血脂及肝脏中脂肪(粗)含量的影响。方法用Wistar大鼠建立高脂血症动物模型,用酶学的方法观察各组大鼠血清中甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-c)及高密度脂蛋白胆固醇(HDL-c)含量的变化。用索式法测定各组大鼠肝脏中脂肪(粗)含量。结果沙苑子水提取液、油提取液、50％乙醇提取液和95％乙醇提取液大剂量(90g／kg)使用15d,可显著降低实验性高脂血症大鼠血中TC、TG的含量及肝脏中脂肪(粗)的含量,水提液小剂量组(20g／kg)和油提液中剂量组(45g／kg)可显著升高血清中HDL-c的含量,其作用优于对照药物绞股蓝总甙组,与高脂模型组比较有极显著性差异(P＜0.001)。结论沙苑子提取液有降低实验性高脂血症大鼠血清中TG、TC、LDL-c的含量和升高HDL-c作用;同时可降低高脂血症大鼠肝脏中脂肪(粗)含量。

Objective To observe the effect of Semen Astragali Complanati extracts on lipid metabolism in rats with hyperlipermia, including blood fat and fat content in liver. Methods The changes of triglyeride （TG）, total cholesterol（ TC）, low-density lipoprotein-c （LDL-c） and high density lipoprotein-c （HDL- c） in serum of Waster rats with hyperlipermia were observed by enzymatic method. The fat content in the liver was detected by soxhlet extraction. Results TC, TG and fat content in the liver of hyperlipermia model rats were significantly decreased after water extract, oil extract, 50% alcohol extract and 95% alcohol extract of Semen Astragali Complanati treating for 15 days （90g/kg）. HDL-c could be increased in the low dose of water extract group （20 g/kg） and the middle dose of oil extract group （45 g/kg）, and these effects were more significant than those in the gypenosides positive control group. There was significant difference between the treating group with the model group （ P 〈 0. 001 ）. Conclusion Extracts of Semen Astragali Complanati have the effect of decreasing TG, TC, LDL-c and fat content in the liver and increasing HDL-c of hyerlipermia model rats.