电针血清对体外培养星形胶质细胞活性的影响

Effect of electroacupuncture stimulation-derived serum on astrocytes activity in vitro

目的探讨电针血清对星形胶质细胞增殖活力及分泌脑源性神经营养因子(BDNF)的影响。方法采用100 Hz高频电针针刺正常大鼠百会、风府两穴,电针2周后采血制备电针血清,加入体外培养的星形胶质细胞培养基。MTT法检测电针血清对星形胶质细胞活力的影响,免疫组化技术显示星形胶质细胞BDNF表达。结果电针血清能够促进星形胶质细胞增殖,但其作用无明显量效关系,在观察范围内,10％电针血清为最佳效应浓度;电针组细胞培养时间延长,星形胶质细胞活力增加,二者增长呈平行关系,48 h电针组OD值为0.372;10％电针血清培养星形胶质细胞5d后BDNF阳性细胞数较对照组明显增多。结论电针血清能够激活星形胶质细胞,促进星形胶质细胞增殖,激活的星形胶质细胞通过分泌BDNF发挥对神经元的保护作用。

Objective To investigate the effect of the serum collected from rats with electroacupuncture stimulation on the proliferation and the expression of brain-derived neurotrophic factor （BDNF） of astrocytes. Methods Baihui （GV20）, fengfu （GV16） were stimulated with 100Hz high frequency electroacupuncture in rats. Serum was collected two weeks later from the stimulated rats and added into the culture medium of astrocytes. Cell viability was detected by MTI＂ assay and the expression level of BDNF was tested by immunohistochemistry. Results The serum derived from the rats stimulated by electroacupuncture could promote the activity of astrocytes, but it was not in an obvious dose-dependent manner. The optimal concentration of the serum was 10%. The cell viability of astrocytes increased in a time-dependent manner with the peaking OD value of 0.372 48hours after the cells were cultured in the medium containing the 10% serum. BDNF expression in astrocytes increased significantly 5 days after astrocytes were treated by the 10% serum. Conclusion The serum from electroacupuncture stimulated rats could promote the activity of cultured astrocytes and enhance the astrocytes proliferation. The activated astrocytes pretect neuron by BDNF secretion.