雄激素受体在脑膜瘤中的表达及其临床意义

Gene and protein expression of androgen receptor in meningioma and its clinical significance

目的观察33例脑膜瘤患者肿瘤组织中雄激素受体(AR)的表达,探讨AR的表达与脑膜瘤临床病理的相关性。方法应用免疫组化SP法及原位杂交法检测脑膜瘤组织标本中AR的表达情况。结果AR在恶性脑膜瘤组中的染色强度明显高于良性脑膜瘤组,其平均积分光密度值(IOD)分别是25.41±13.42和6.20±4.75(P<0.05);AR mRNA的IOD分别为19.46±9.33和4.72±4.68(P<0.05)。结论AR在脑膜瘤的表达与肿瘤细胞的病理性质(良、恶性)有关,AR可作为鉴别良恶性脑膜瘤的辅助诊断指标。

[Objective] To investigate androgen receptor（AR） expression in meningiomas,and the correlation between AR expression and clinical pathology tissue grading. [Methods] 33 samples from different patients were collected. AR expression each sample was measured by HE staining, in situ hybridization （ISH） and immunohistochemistry （IHC） staining. [Results] The Staining intensity of AR protein was significant higher in malignant group than that in benign group. The average integral optical densities （IOD） of AR protein in malignant and benign groups were 25.41 ± 13.42 and 5.19 ± 2.63 respectively （ P 〈 0.01） ; The IOD of AR mRNA were 19.46 ± 9.33 and 4. 72±4.68 respectively（ P〈 0.05）. [Conclusions] The expression of AR in meningioma is related with benignancy and malignance of tumour cells in clinical pathology. The expressions of AR can help clinical differential diagnosis as a useful adjuvant marker.