MDR1基因G2677T／C3435T多态性对鼻咽癌放疗疗效的影响

Effect of G2677T/C3435T genetic polymorphisms of MDR1 gene on radiation response of nasopharyngeal carcinoma

目的探讨多药耐药基因(MDR1)21外显子G2677T和26外显子C3435T多态性对鼻咽癌根治性放疗疗效的影响。方法采用PCR和限制性片断长度多态性(RFLP)对59例鼻咽癌患者行MDR1基因分型,并用测序法验证。结果G2677T GG基因型携带者行根治性放疗的效果优于GT和TT基因型,但差异无显著性;C3435T CC基因型携带者放射敏感性显著强于CT和TT基因型携带者(P=0．026)。结论MDR1基因多态性可作为鼻咽癌患者放射敏感度的遗传学标志。

[Objective] To evaluate the effect of G2677T/C3435T genetic polymorphisms of multidrug resistance gene （MDR1） on radiation response of nasopharyngeal carcinoma （NPC）. [Methods] Blood samples were collected from 59 NPC patients devided into two groups （radiosensitive or radioresistant） according to the effects of radiation therapy. The genotypes for MDR1 polymorphisms （G2677T in exon 21 and C3435T in exon 26） were determined by PCR and restriction fragment length polymorphism analysis. The results were further confirmed by sequencing, [Results] The G2677T GG genotype was associated with a better radiotherapy response compared with the combined GT and TT genotypes, although it was not statistically significant. The C3435T CC genotype was associated with a significantly better radiotherapy response compared with the combined CT and TT genotypes （P = 0. 026）. [Conclusions] The MDR1 polymorphisms can be used for predicting the response to radiotherapy in NPC patients as genetic markers,