巨噬细胞在血管重塑过程中的作用

The role of macrophages during vascular remodeling

目的:探讨体内巨噬细胞对平滑肌细胞增殖与迁移的影响。方法:采用兔后肢股动脉—静脉短路诱导侧枝血管生长模型。一周后将动物处死,应用Ki67(细胞增殖标记物)免疫荧光组织化学技术和弹性纤维组织化学染色技术,在连续切片上检测髂外动脉在重塑过程中巨噬细胞数目和平滑肌细胞增殖及弹性纤维的降解。结果:在重塑血管外膜发现大量巨噬细胞,中膜也有大量巨噬细胞的存在,平滑肌细胞排列紊乱;有大量的平滑肌细胞增殖。在正常血管横断面上中膜有大量环形排列的弹性纤维存在,重塑的血管巨噬细胞聚集和平滑肌细胞增殖的部位弹性纤维发生明显降解。结论:血管重塑过程中,侵入血管壁的巨噬细胞可能通过分泌生长因子和细胞基质降解酶促进细胞外基质的降解,刺激平滑肌细胞增殖与移动,参与血管重塑的调节。

Objective： To investigate the involvement of macrophages in the proliferation and migration of smooth muscle cells in vascular remodeling. Mothoda： The model of arteriogenesis induced by artery-vein shunt in rabbit was used. The animals were killed after survival for one week, then the remodeling of iliac arterial vessels were studied in a serial sections by elastica van Gieson staining and confocal immunofluorescence using specific antibodies against RAM11 （macrophage marker） and ki67 （cell proliferation marker）. Results： A large amount of macrophages were found accumulated in the adventitia in the remodeling vessels, also presented in the tuniea media. Thesmooth muscle cells irregularily arranged. In the regions where macrophages were accumulated, the proliferation of smooth muscle cell was evident, and the degradation of elastic fibers was also found. Conclusions： The data suggests that the invaded macrophages mediate vascular remodeling during arteriogenesis through producing growth factors and the enzymes for degradation of extracellular matrix to faciliate cell prolferation and migration.