自身免疫病患者造血干细胞移植后Ts及Tc细胞的变化

Alteration of Ts and Tc lymphocytes subsets in patients with serious autoimmune disease after autologous hematopoietic stem cell transplantation

目的检测严重自身免疫病（SAID）患者自体造血干细胞移植（AHSCT）前后外周血中抑制性T（Ts）细胞、杀伤性T（Tc）细胞亚群的变化,探讨Ts、Tc细胞在AHSCT诱导自身免疫病缓解中的作用。方法使用流式细胞术（FCM）检测对照组及SAID患者AHSCT前1周及AHSCT后1-3月外周血中Ts、Tc细胞亚群。Ts细胞表型为CD3^＋CD8^＋CD28^-,Tc表型为CD3^＋CD8^＋CD28^＋。分析各细胞亚群占T淋巴细胞的百分率,并比较AHSCT前后Ts、Tc的变化。结果SAID患者AHSCT前Ts与对照组比较差异无统计学意义（P〉0.05）,Tc高于对照组（P〈0.05）。AHSCT前后比较,患者外周血中Ts细胞显著增高（P〈0.05）;Tc虽有下降,但差异无统计学意义（P〉0.05）。AHSCT后与对照组比较,Ts显著增高（P〈0.05）,Tc差异无统计学意义。结论AHSCT治疗SAID患者,能显著提高患者Ts细胞,降低Tc细胞的比例,是AHSCT诱导SAID缓解的机制之一。

Objective To study the mechanism of remission on patients with serious autoimmune disease （SAID） induced by autologous hematopoietic stem cell transplantation（AHSCT） by an analysis of the alteration in CD8 ^＋ CD28^- suppressor（Ts） lymphocytes and CD8 ^＋ CD28 ^＋ cytotoxic （Tc） lymphocytes subsets in peripheral blood before and afetr AHSCT. Methods Ts and Tc subsets in peripheral blood were examined by flow cytometry （FCM）. Peripheral blood was obtained from patients with SAID one week before AHSCT and one to three month after AHSCT. The cells whose phenotype were CD3^ ＋ CD8 ^＋ CD28 ^- were considered Ts. The cells whose phenotype were CD3^ ＋ CD8^ ＋ CD28^ ＋ were considered Tc. The percentages of each group of the cells were examined. Results There was no difference in the percentages of Ts between patients and controls （ P 〉 0.05 ）. The percentage of Tc was increased in patients （ P 〈 0.05 ）. After AHSCT,the percentage of Ts in patients with ASID was significantly higher than that before AHSCT and in controls. There was no difference in the percentages of Tc in patients with SAID after AHSCT compared with that before AHSCT and in controls（P 〉 0.05）. Conclusions After AHSCT,the level of Ts subset is higher and the level of Tc subset is lower compared with normal controls. It is helpful to remission in patients with SAID.