应用全自动生化分析仪测定缺血修饰白蛋白

Detection of ischemia modified albumin using automatic biochemical analyzer

目的建立白蛋白钴结合(ACB)试验检测缺血修饰白蛋白(IMA)。方法应用日立7600型全自动生化分析仪,采用ACB法测定IMA,并测定血清中不同白蛋白浓度,以观察白蛋白对其影响。结果ACB方法批内变异系数(CV)为5.0%,批间CV为1.6%。不同浓度的人血清白蛋白ACB法测定结果是线性的,回归方程为Y=0.029+1.57X,r=0.995,P=0.000。黄疸对测定无明显影响;溶血(5 g/dL)和脂血(0.4%)对测定有干扰。健康人群参考范围为79.6 U/mL。结论IMA是评价心肌缺血的有效诊断工具,采用全自动生化分析仪建立测定IMA的方法方便、简单,可以大批量测定。

Objective To establish a method detecting ischemia modified albumin（IMA） in serum sample. Methods IMA was determined by the albumin cobalt binding（ACB） test using the Hitachi 7600 automatic biochemical analyzer. Serum abumin was also determined. Results Coefficient of variation （CV） for within-run assays was 5.0%, CV for between-run assays was 1.6%. Measurement of ACB using different concentrations of human serum albumin was linear, regression equation was Y = 0. 029 ＋ 1.57X,r = 0. 995, P = 0. 000. There was no significant interference observed for bilirubin. Hemoglobin （5 g/dL） and triglycerides （0.4%） had interference on the ACB test. Healthy individuals reference value was 79.6 U/mL. Conclusions IMA may be an effective biomarker for detecting myocardial ischemia. It is a very convenient to detect IMA on automatic biochemical analyzer.