系统性红斑狼疮患者血清可溶性细胞间粘附分子-1、L-选择素、细胞因子的变化及意义

Changes and significance of serum soluble ICAM-1,L-selectin,IL-2,IL-6,IL-8 and IL-10 in patients with systemic lupus erythematosus

目的探讨系统性红斑狼疮(SLE)患者血清可溶性细胞间粘附分子-1(sICAM-1)、L-选择素、白细胞介素(IL)-2、IL-6、IL-8和IL-10水平的变化及其致病意义。方法采自24例SLE患者的血清标本分别用酶联免疫吸附试验(ELISA)检测sICAM-1和L-选择素;用放射免疫检测法(RIA)检测IL-2、IL-6、IL-8和IL-10;另以20名健康志愿者作为对照。结果SLE患者血清sICAM-1、L-选择素、IL-6及IL-2/IL-10比值显著高于对照组(P<0.05),IL-2无明显变化,IL-8水平稍高于对照组,但差异无统计学意义(P>0.05)。结论SLE患者sICAM-1、L-选择素和IL-6表达增加,而IL-10表达减少,提示免疫紊乱、促炎与抗炎细胞因子失平衡参与了SLE的发病过程。

Objective To evaluate the changes and significance of serum soluble intercellular adhesion molecule-1 （sICAM-1）, L-selectin, interleukin （IL）-2, IL-6, IL-8 and IL-10 in patients with systemic lupus erythematosus （SLE）. Methods sICAM-1 and L-selectin were detected by enzyme-linked immunosorbent assay （ELISA） and IL-2, IL-6, IL-8 and IL-10 by radioimmunoassay （RIA） in the serum samples from 24 SLE cases and 20 healthy volunteers. Results Compared with controls, the levels of serum sICAM-1, L-selectin, IL-6 and IL-2/IL-10 ratio in SLE patients were significantly increased （P 〈 0.05 ）. However there was no significant difference in IL-2 levels between two groups, and the level of IL-8 was only slightly higher in SLE patients than in healthy volunteers （ P 〉 0.05 ）. Conclusions Patients with SLE expressed much more sICAM-1, L-selectin and IL-6. It strongly suggersted that immune dysfunction and pro-/anti-inflammatory imbalance would contribute to the pathogenesis of SLE.