纤维素―刚果红培养基鉴定产纤维素酶真菌的机理研究

Study on Mechanism of Cellulose-Congo-Red Medium in Identificating Cellulase-producing Fungi

对产纤维素酶真菌F160和不产纤维素酶阴性对照菌毛霉在纤维素―刚果红培养基中刚果红染料移动情况进行研究,结果表明刚果红染料进入产纤维素酶真菌的机理为,产纤维素酶真菌首先分解纤维素物质为含有葡聚糖等结构的多聚糖类物质,多聚糖与刚果红形成多聚糖―刚果红复合物,复合物不仅被吸附在菌丝外,而且能被进一步转运吸收至菌丝内部。通过进一步的降解,多聚糖被分解而加以利用,而刚果红则被保留在菌丝体内,使菌落呈现红色。所以,纤维素―刚果红培养基可作为分离、筛选纤维素分解真菌的特异性培养基。

Transition of congo-red in cellulose congo-red medium was studied by cellulaseproducing fungi F160 and no-cellulase-producing mucor. Results showed that cellulose was firstly decomposed and turned into polysaccharides by cellulose-producing fungi, which possessed of glucans structure, then polysaccharides bonded congo-red to form polysaccharides-congo red complex. The complex was not only adhered on the surface of hyphae, but also absorbed inside them, and decomposed to be glucose, which could be utilized by fungi easily. And that, congo red deposited in hypha, colored the colony red. So, cong-red medium can be used as a special medium to identify fungi oossessing cellulase.