增殖细胞核抗原和p16蛋白表达与鼻咽癌放射敏感性相关性研究

Relationship between the expression of PCNA and p16 protein and the radiosensitivity of nasopharyngeal carcinoma

目的探讨增殖细胞核抗原（PCNA）和p16蛋白表达与鼻咽癌放射敏感性的关系。方法应用免疫组化法,检测63例初行放疗前鼻咽癌组织中PCNA和p16蛋白表达情况。结果63例鼻咽癌组织中PCNA和p16蛋白表达阳性者分别有62例（98.41%）和13例（20.63%）,高表达者分别有22例（34.92%）和7例（11.11%）;鼻咽癌组织中PCNA表达与p16蛋白表达呈负相关（rs=-0.79,P〈0.01）;PCNA及p16蛋白阳性表达水平越高,则鼻咽癌对放射治疗越敏感,放疗后鼻咽局部癌肿完全消退率越高（P〉0.05）;PCNA和p16蛋白同时高表达者比单一指标高表达者,对放射敏感性更高（P〈0.05）。结论PCNA和p16蛋白是反映鼻咽癌放射敏感性和预测其放疗疗效的重要指标,在作为放疗敏感性的指标上,PCNA可能较p16更有意义。

Objective To investigate the relationship between the expression of PCNA and p16 protein and the radiosensitivity of nasopharyngeal carcinoma （NPC）. Methods PCNA and p16 protein expression were detected by immunohistochemistry method in 63 biopsy specimens before radiotherapy of NPC. Results The rates of PCNA and p16 protein expression were 98.41% （62/63） and 20. 63 % （ 13/ 63） respectively. The rates of PCNA and p16 protein high expression were 34. 92% （22/63） and 11.11% （7/63） respectively. The expression of PCNA and p16 protein were hightly correlated （ r, = - 0. 79, P 〈 0. 01 ） . The higher the expression of PCNA and p16 protein were, the higher the radiosensitivity of NPC was, and the better the curative effect was（ P 〈0. 05）. Furthermore the radiosensitivity of NPC was higher in the cases with high expression of beth PCNA and p16 protein than that in the cases with the high expression of either PCNA or p16 protein （ P 〉 0. 05 ） ,Conclusion PCNA and p16 protein were the important indicators for the radiosensitivity of NPC and the effect of radiotherapy.