蛇毒抗高凝状态酶对种植肝癌H22小鼠肿瘤抑制作用的研究

Preliminary Study of AHCSE on Anticancer Effect of Experimental Liver Cancer (H22) in Mice

目的探讨蛇毒抗高凝状态酶(AHCSE)对实验小鼠肿瘤的抑制作用。方法20只Balb/c小鼠随机分为治疗组和对照组,左前肢腋下接种H22肝癌瘤组织,治疗组腹腔途径注射AHCSE 2 mg/kg体重,对照组用生理盐水代替。结果对照组瘤结较大,显微镜下见瘤细胞生长活跃、核大、核仁明显、核分裂多见,而坏死灶少见,且瘤细胞向周围浸润扩散;治疗组瘤体较小,瘤细胞固缩、核仁不明显、核分裂少见,而坏死灶多见,瘤细胞周围有少量纤维组织增生。结论AHCSE具有显著的体内抑制肿瘤生长的作用。

Objective To observe the pathological change of antihypercoagulability state enzyme of snake venom（AHCSE） on experimental liver cancer in mice. Methods BALB/C mice liver cancer cells （H22） were separatedly inoculated hypodermically in mice. Then these mice were killed thirteen days later. Tool the tumor modules in killing mice observed and pathological study. Results In control group the tumor nodules were rather large and the focus of necrosis was not evident; under the microcope the cells grew actively, nucleolic were obvious, mitosis were frequent and tumor cells infitrated and spred to adjacent regions. In the therapy group, the tumor noduls were rather small and the focus of necrosis was rather large; under the microscope the tumor cells were pyknosis and nucleolic were no evident. Mitosis were not frequent and had more necrosis focus, and the tumor cells were surrouded by some hyperplasia fibrous tissue. Conclusions AHCSE on anticancer effect in mice was＇ evident.