激素合并内毒素诱导大鼠股骨头坏死扫描电镜观察

Observing on Avascular Necrosis of the Femoral Head Induced of Rat by Glucocorticoid Combining with Endotoxin by Scanning Electron Microscope

目的以扫描电镜观察大鼠激素合并内毒素诱导股骨头缺血性坏死模型病理变化,为实验和临床研究提供标准化动物模型。方法雄性SD大鼠8只,尾静脉注射大肠埃希氏杆菌脂多糖(LPS,Lipopolysaccharide)10μg/kg(wt)一次,肌肉注射甲基强的松龙20 mg/kg(wt)三次。另2只为空白对照。6周后处死,取股骨头,扫描电镜观察。结果造模大鼠股骨头表面失去小丘样结构,不光滑并有塌陷,呈蜂窝状改变。结论糖皮质激素合并内毒素可诱导出大鼠股骨头坏死早期病理变化。

Objective To observe rat avascular necrosis of the femoral head （ANFH） induced by glueocortieoid combining endotoxin by scanning electron microscope and investigate the standard animal model of ANFH. Method 8 male SD rat were injected LPS 10 μ/kg（wt） in vena eaudalis once, and methylprednisolone 20 mg/kg（wt） i.m. 3 times. 2 rats were controlled. All rat were killed after 6 weeks and the femoral heads were observed by scanning electron microscope. Result The surfaces of femoral head were collapsing, not smooth, missing the nodule structure and honeycomb appearance. Conclusion Glucocorticoid combining endotoxin can induce the initial stage pathological change of ANFH rat model.