雷公藤多甙对实验性自身免疫性脑脊髓炎的治疗及炎症浸润细胞凋亡的影响

Therapy of experhnental autoinnmme encephalomyefitis by Tripterygimn wifordii polyglycosidium and the effects on apoptosis of inflannnatory infiltrating cells

目的 观察雷公藤多甙（TWP）能否抑制实验性自身免疫性脑脊髓炎（EAE）模型的发病、病变程度以及对中枢神经系统（CNS）炎症浸润细胞凋亡的影响，为临床应用提供实验依据。方法将雌性豚鼠随机分为对照组、EAE组和喂服TWP组，EAE组和TWP组豚鼠采用颈背部皮下多点注射完全弗氏佐剂-豚鼠全脊髓匀浆（CFA-GPSCH），并辅以注射百日咳疫苗（BPV），诱导豚鼠建立EAE模型。观察各组EAE的发病情况，在光学显微镜下计数中枢神经系统炎症细胞浸润程度，并用3’末端脱氧核糖核苷酸转移酶（TdT）介导的dUTP缺口末端标记（TUNEL）检测其凋亡情况。结果 TWP治疗组豚鼠发病数较EAE对照组减少，起病时间相对延迟，临床表现明显减轻，炎性细胞浸润明显减少。与EAE对照组相比，TWP治疗组的中枢神经系统炎症浸润细胞的凋亡率明显增高。结论 TWP可有效地抑制EAE的发病，其机制可能与其抑制中枢神经系统炎症细胞的浸润及增加炎症细胞的凋亡有关，提示该药有临床应用前景。

Objective To observe the influence of Tripterygium wifordii polyglycosidium （TWP） on the severity and incidence of experimental autoimmune encephalomyelitis （EAE） and on the apoptosis of inflarmnatory infiltrating cells in the central nervous （CNS）. Methods Female guinea pigs were randomly divided into control group, EAE group and TWP group. EAE models were established in the EAE group and TWP group by injecting guinea-pig spinal cord homogenate （GPSCH） in complete Freund＇s adjuvant （CFA） in four locations on the back of the guinea-pig neck and injecting bordetella pertussis vaccine （BPV） in one footpad.Then the guinea pigs were observed,inflammatory infiltrating cells in the CNS were counted under the microscope and the apoptosis of them was tested by terminal deoxynucleotidyl transferase mediated dUTP nick end labehng （TUNEL）.Results Compared with the EAE group, the TWP group had lower incidence,delayed onset,alleviated clinical appearance and inflammatory infihration.Compared with the EAE group,the apoptotic rate of inflammatory infiltrating cells in the TWP group was significantly increased. Conclusion TWP could effectively inhibit the incidence of EAE, and the mechanism might be associated with the inhibition of infiltration and the promotion of apoptotic rate of inflammatory ceils in the CNS, which suggests the promising perspective of TWP in the clinical apphcation.