人巨细胞病毒先天性潜伏感染老龄小鼠肺组织的实验研究

An experimental study on the lung of aged mice with hmnan cytomegalovirus congenital latent infection

目的 观察HCMV先天性潜伏感染老龄小鼠肺组织的病理改变，为进一步研究HCMV潜伏感染再激活与肺部疾病的关系提供初步依据。方法HCMV AD169株感染BALB／c小鼠雌雄配对，所生子鼠饲养18个月后随机取6只腹腔注射环磷酰胺为病毒激活组，另取6只为病毒潜伏组；同样方法设立DMEM激活组和DMEM未激活组为对照。取各组小鼠肺组织进行病毒分离与鉴定、PCR和RT-PCR检测HCMVUL83基因及其转录产物、HE染色等。结果 病毒激活组小鼠肺组织病毒分离、PCR及RT-PCR均为阳性；HE染色可见肺泡间隔增宽，有大量炎性细胞浸润，肺泡腔有渗出，可观察到HCMV特征性包涵体。病毒潜伏组仅PCR为阳性，其余均为阴性。对照组小鼠所有结果均为阴性。结论HCMV AD169株可引起小鼠先天性肺组织潜伏感染，并在免疫抑制剂作用下可能激活转变为活动性感染造成肺组织病理损伤。

Objective To study the HCMV congenital latent infection and reactivation from latency in mice, and to observe pathologic change of pulmonary tissue. Methods Germ-frce BALB/c mice（half are female） were infected with 10^5 PFU/ml of HCMV by intraperitoneal and arranged for mating. Offsprings surviving were maintained for 18 months in isolated cages. Twelve mice were selected randomly, half of which were injected with cyclophosphamide as the group of activation, others as the group of latency. At the same time, the negative controls were set up by same method. Lungs were harvested for virus isolation,PCR,RT-PCR and stained by HE.Resuits（1） Virus isolation：there was no preductive virus particles in all mice before reactivation, while virus particles could be detected in the lung of mice in activation group.（2） By indirect inmmunofluorescent, HCMV pp65 were detected in the cell slice of activation group.（3） By HE staining,HCMV specific inclusion bodies could be observed in lung tissue sections of mice in activation group.（4） The UL83 gene was detected in the lung of mice in latency group, while both the gene and corresponding transcripts could be detected in the lung of mice in activation group. Results of control group were negative. Conclusion Reactivation of HCMV from latency can induce pathologic change of pulmonary tissues.