摘要
根癌农杆菌介导的丝状真菌遗传转化是近年建立起来的一种新方法,该方法和以往的真菌转化体系相比具有转化方法简单、材料易得、效率高以及转化子中T-DNA单拷贝插入比例高等特点。就根癌农杆菌转化的丝状真菌种类、转化的具体过程以及影响转化效率的因素等方面进行了综述,并展望了该方法的应用前景。
Agrobacterium tumefacienz mediated transformation （ATMT）was newly used to transform filamentous fungi.Because of the simplicity and efficiency of transformation via A.tumefaciens ,it is relatively easy to generate a large number of stable transformants.In combination with the finding that the T-DNA integrates randomly and predominantly as a single copy,AMT is well suited to perform insertional mutagenesis in fungi.This paper summarized the advancement of ATMT and discussed the future application of this technology in filamentous fungi.
出处
《生物技术通报》
CAS
CSCD
2007年第3期111-114,共4页
Biotechnology Bulletin
关键词
根癌农杆菌
丝状真菌
遗传转化
T-DNA
Agrobacterium tumefaciens Filamentous fungi Transformation T-DNA
